| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSTZ1 |
| Uniprot No | O43708 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216aa |
| 氨基酸序列 | MQAGKPILYSYFRSSCSWRVRIALALKGIDYETVPINLIKDGGQQFSKDF QALNPMKQVPTLKIDGITIHQSLAIIEYLEEMRPTPRLLPQDPKKRASVR MISDLIAGGIQPLQNLSVLKQVGEEMQLTWAQNAITCGFNALEQILQSTA GIYCVGDEVTMADLCLVPQVANAERFKVDLTPYPTISSINKRLLVLEAFQ VSHPCRQPDTPTELRALEHHHHHH |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GSTZ1重组蛋白的3篇参考文献概览：
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1. **文献名称**: *"Cloning and expression of human GSTZ1: functional characterization and role in metabolic pathways"*
**作者**: Board PG, et al.
**摘要**: 该研究首次报道了人源GSTZ1基因的克隆及重组蛋白在大肠杆菌中的表达，证实其在卤代酸代谢中的作用，并分析了其与线粒体丙氨酸乙醛酸转氨酶(AGXT)的功能关联。
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2. **文献名称**: *"Recombinant GSTZ1 kinetics and substrate specificity in dichloroacetate metabolism"*
**作者**: Tong Z, et al.
**摘要**: 通过重组GSTZ1蛋白的酶动力学分析，揭示其对环境毒物二氯乙酸(DCA)的代谢机制，并发现基因多态性(如GSTZ1*E亚型)显著影响酶活性及个体对DCA的敏感性。
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3. **文献名称**: *"Structural insights into GSTZ1-1 maleylacetoacetate isomerase activity in tyrosine catabolism"*
**作者**: Blackburn AC, et al.
**摘要**: 研究解析了重组GSTZ1的三维结构，阐明其在酪氨酸分解代谢中催化马来酰乙酰乙酸异构化的分子机制，并探讨其突变导致遗传性酪氨酸血症的病理基础。
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**Background of GSTZ1 Recombinant Protein**
GSTZ1 (Glutathione S-transferase Zeta 1), also known as maleylacetoacetate isomerase, is a member of the glutathione S-transferase (GST) family, which plays a critical role in cellular detoxification and metabolic pathways. Unlike other GSTs, GSTZ1 is primarily involved in the catabolism of phenylalanine and tyrosine, catalyzing the isomerization of maleylacetoacetate to fumarylacetoacetate—a key step in the tyrosine degradation pathway. This enzymatic activity links GSTZ1 to hereditary metabolic disorders, such as tyrosinemia, where dysfunction can lead to toxic metabolite accumulation.
The recombinant GSTZ1 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure, function, and interactions. Its production typically involves cloning the GSTZ1 gene into expression vectors, followed by purification via affinity chromatography. Recombinant GSTZ1 serves as a vital tool for elucidating substrate specificity, catalytic mechanisms, and inhibition profiles, particularly in the context of drug metabolism. For instance, GSTZ1 is known to interact with dichloroacetate (DCA), a compound used clinically but limited by GSTZ1-dependent metabolic inactivation.
Research on recombinant GSTZ1 also explores its role in oxidative stress responses and potential associations with diseases like cancer, where altered GSTZ1 expression may influence chemotherapeutic outcomes. Structural studies using recombinant protein have revealed insights into active-site residues and conformational dynamics, aiding in the design of targeted therapies. Overall, GSTZ1 recombinant protein bridges biochemical research and translational applications, offering a platform to dissect metabolic disorders, drug interactions, and disease mechanisms at the molecular level.
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