| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RAB5 |
| Entrez GeneID | 5868 |
| clone | 3H5C11 |
| WB Predicted band size | 23.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Rab5a (AA: 1-215) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下为3篇与Rab5a抗体相关的示例文献(注：以下信息为模拟示例，实际引用请以真实文献为准)：
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1. **标题**: "Rab5a regulates endosome fusion and early signal transduction in immune cells"
**作者**: Smith J, et al.
**摘要**: 研究通过Rab5a抗体进行免疫沉淀和Western blot分析，揭示Rab5a在巨噬细胞内体融合中的关键作用，并证明其通过调控EGFR信号通路影响细胞迁移。
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2. **标题**: "Rab5a overexpression promotes tumor invasion via vesicle trafficking in breast cancer"
**作者**: Chen L, et al.
**摘要**: 利用Rab5a抗体的免疫荧光技术，发现Rab5a在乳腺癌细胞中高表达，通过增强MMP-9的囊泡运输促进细胞外基质降解，导致转移能力增强。
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3. **标题**: "Rab5a dysfunction alters amyloid-β processing in Alzheimer's disease models"
**作者**: Wang Y, et al.
**摘要**: 通过Rab5a抗体标记小鼠神经元内体，证实Rab5a活性异常会扰乱淀粉样前体蛋白(APP)的运输，加剧Aβ肽段积累，为阿尔茨海默病机制提供新见解。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“Rab5a antibody”+研究领域(如癌症、神经科学等)获取近期论文。
Rab5a antibody is a crucial tool in studying the molecular mechanisms of intracellular vesicular trafficking. Rab5a, a member of the Rab GTPase family, plays a pivotal role in regulating early endosome fusion, endocytic transport, and membrane dynamics. It functions as a molecular switch, cycling between active GTP-bound and inactive GDP-bound states to coordinate cargo sorting, vesicle formation, and organelle maturation. Dysregulation of Rab5a has been implicated in various pathologies, including cancer metastasis, neurodegenerative disorders, and viral infections.
Rab5a antibodies are specifically designed to detect and quantify Rab5a protein expression in experimental models. They enable researchers to investigate Rab5a's subcellular localization via immunofluorescence, assess protein levels through Western blotting, and explore protein-protein interactions using co-immunoprecipitation. These antibodies are typically developed in host species like rabbits or mice, targeting conserved epitopes in human, mouse, or rat Rab5a isoforms. Validation parameters include specificity checks using knockout cell lines and functional assays confirming antibody performance in relevant applications.
As a marker for early endosomes, Rab5a antibodies are widely used in cell biology research to dissect endocytic pathways, autophagy, and receptor trafficking. Recent studies also utilize them to explore Rab5a's role in immune responses, synaptic vesicle recycling, and extracellular vesicle biogenesis, underscoring their versatility in both basic and translational research.
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