Mouse Monoclonal SH3GL1 Antibody

中文名： SH3GL1抗体
别名： EEN; CNSA1; SH3P8; SH3D2B

货号: IPD30945

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	咨询技术	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	1/200 - 1/400	Human,Mouse,Rat
Elisa	1/10000	Human,Mouse,Rat

产品详情

Aliases	EEN; CNSA1; SH3P8; SH3D2B
Entrez GeneID	6455
clone	2A9H4
WB Predicted band size	41.5kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human SH3GL1 (AA: 12-119) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Flow cytometric analysis of Hela cells using SH3GL1 mouse mAb (green) and negative control (red).

参考文献

以下是关于SH3GL1抗体的3篇参考文献示例(文献信息为模拟概括，仅供参考)：

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1. **文献名称**: "SH3GL1 regulates endocytic membrane trafficking through its role in actin polymerization"

**作者**: Smith A, et al.

**摘要**: 该研究利用SH3GL1特异性抗体，发现SH3GL1通过调控肌动蛋白动态变化参与网格蛋白介导的内吞作用，揭示了其在细胞膜重塑中的关键功能。

2. **文献名称**: "Characterization of a monoclonal antibody against SH3GL1 for detecting neurodegenerative disease biomarkers"

**作者**: Lee J, et al.

**摘要**: 研究开发了一种高特异性SH3GL1单克隆抗体，验证了其在脑组织样本中的免疫组化及Western blot应用，并发现SH3GL1表达水平在阿尔茨海默病模型中显著下调。

3. **文献名称**: "SH3GL1 interacts with EGFR and modulates cancer cell proliferation"

**作者**: Chen X, et al.

**摘要**: 通过SH3GL1抗体的免疫共沉淀实验，证实SH3GL1与表皮生长因子受体(EGFR)结合，抑制其下游信号通路，从而抑制肿瘤细胞的异常增殖。

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注：上述文献为示例，实际研究中建议通过PubMed或Google Scholar检索具体文献(关键词：SH3GL1/Endophilin-A2 + antibody)。如需真实文献，可提供进一步信息协助查询。

背景信息

SH3GL1 (SH3 domain-containing GRB2-like 1), also known as EEN-B1 or CNSA1. is a member of the Bin-Amphiphysin-Rvs (BAR) domain protein family. It plays a role in membrane trafficking, endocytosis, and synaptic vesicle recycling by facilitating membrane curvature and vesicle formation. The SH3GL1 antibody is a crucial tool for studying its expression, localization, and function in cellular processes. This antibody specifically recognizes the SH3GL1 protein, which contains an N-terminal BAR domain for membrane binding and a C-terminal SH3 domain that interacts with proline-rich motifs in partner proteins like endophilins and dynamin.

Research using SH3GL1 antibodies has linked the protein to clathrin-mediated endocytosis, receptor tyrosine kinase signaling (e.g., EGFR degradation), and neuronal communication. Its involvement in membrane dynamics makes it relevant to cancer research, particularly in tumorigenesis and metastasis, where altered endocytic trafficking may influence cell signaling. In neuroscience, SH3GL1 antibodies help investigate synaptic vesicle recycling defects associated with neurodegenerative diseases.

Commercially available SH3GL1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression levels or subcellular distribution. Validation typically includes testing in knockout cell lines or tissues to ensure specificity. Studies employing these antibodies continue to reveal SH3GL1's regulatory roles in cellular homeostasis and disease pathways, emphasizing its potential as a therapeutic target.