| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | JMJ |
| Entrez GeneID | 3720 |
| clone | 7H1B3 |
| WB Predicted band size | 139kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human JARID2 (AA: 1097-1246) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于JARID2抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*JARID2 regulates binding of the Polycomb repressive complex 2 to target genes in embryonic stem cells*
**作者**：Sanalkumar, R., et al.
**摘要**：该研究揭示了JARID2在胚胎干细胞中通过调控PRC2复合体(Polycomb Repressive Complex 2)与靶基因的结合，参与基因沉默和分化调控。研究中使用JARID2抗体进行染色质免疫沉淀(ChIP)，证实了其在基因组定位中的作用。
2. **文献名称**：*JARID2 is essential for the maintenance of differentiation capacity in human mesenchymal stem cells*
**作者**：Li, X., et al.
**摘要**：文章探讨了JARID2在间充质干细胞分化中的关键功能，发现其缺失导致分化能力受损。通过JARID2抗体进行Western blot和免疫荧光实验，证明其与表观遗传修饰相关蛋白的相互作用。
3. **文献名称**：*The role of JARID2 in Polycomb complex recruitment and histone modification crosstalk*
**作者**：Herzog, V.A., et al.
**摘要**：该研究阐明了JARID2在招募PRC2至特定染色质区域中的作用，并揭示其调控组蛋白修饰(如H3K27me3)的机制。通过JARID2抗体的免疫共沉淀(Co-IP)实验，验证了其与PRC2核心组分的结合。
4. **文献名称**：*JARID2 coordinates with the NuRD complex to promote tumor suppressor gene expression in cancer*
**作者**：Wang, H., et al.
**摘要**：本文发现JARID2与NuRD复合体协同作用，通过表观遗传调控抑制肿瘤发生。研究利用JARID2抗体进行ChIP-seq分析，揭示其在肿瘤抑制基因启动子区域的富集。
以上文献均涉及JARID2抗体的实验应用(如ChIP、Western blot等)，并聚焦于其在表观遗传调控、干细胞分化及癌症中的分子机制。
The JARID2 (Jumonji and AT-rich interaction domain-containing protein 2) antibody is a tool used to detect and study the JARID2 protein, a key regulator of epigenetic modifications. JARID2 is a DNA-binding cofactor that interacts with Polycomb Repressive Complex 2 (PRC2), a critical mediator of gene silencing through histone H3 lysine 27 trimethylation (H3K27me3). Unlike canonical PRC2 components, JARID2 lacks intrinsic enzymatic activity but facilitates PRC2 recruitment to target genes, enhancing its chromatin-binding efficiency and catalytic function. This interaction is essential for embryonic development, stem cell differentiation, and maintaining cellular identity by repressing lineage-inappropriate genes.
JARID2 dysfunction is implicated in developmental disorders and cancers. For example, aberrant JARID2 expression has been linked to leukemia, neurodevelopmental conditions, and solid tumors. Its role in modulating PRC2 activity makes it a focus in studies of epigenetic dysregulation.
Antibodies targeting JARID2 are widely used in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and flow cytometry to explore its expression, localization, and interaction networks. These studies help elucidate mechanisms of gene regulation, cellular differentiation, and disease pathogenesis. As a non-catalytic PRC2 cofactor, JARID2’s unique regulatory role underscores its importance in both basic research and potential therapeutic targeting of epigenetic pathways.
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