| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | UCPH; BMIQ4; SLC25A8 |
| Entrez GeneID | 7351 |
| clone | 3F1B9 |
| WB Predicted band size | 33.2kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human UCP2 (AA: 1-309) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于UCP2抗体的3-4篇参考文献示例(注：以下内容为模拟生成，非真实文献，仅供参考)：
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1. **文献名称**：*"UCP2 Expression in Colorectal Cancer: Prognostic Implications and Mechanistic Insights"*
**作者**：Zhang Y, et al. (2020)
**摘要**：本研究通过免疫组化(IHC)和Western blot(WB)技术，使用特异性UCP2抗体分析结直肠癌组织中的蛋白表达水平，发现UCP2高表达与患者不良预后显著相关，提示其可能通过调节线粒体氧化应激促进肿瘤进展。
2. **文献名称**：*"Protective Role of UCP2 in Diabetic Cardiomyopathy: Evidence from Knockout Mouse Models"*
**作者**：Smith JL, et al. (2018)
**摘要**：作者利用UCP2基因敲除小鼠模型，结合UCP2抗体进行心肌组织WB和免疫荧光检测，证实UCP2缺失加剧糖尿病小鼠的心肌氧化损伤，而UCP2过表达可通过抑制ROS生成改善胰岛素敏感性。
3. **文献名称**：*"UCP2 Modulates Neurodegeneration via Antioxidant Pathways in Alzheimer's Disease"*
**作者**：Kumar R, et al. (2021)
**摘要**：该研究通过UCP2抗体检测阿尔茨海默病模型小鼠脑组织，发现UCP2表达上调与神经元氧化损伤减少相关，进一步机制研究表明UCP2通过激活Nrf2通路发挥神经保护作用。
4. **文献名称**：*"Validation of Commercial UCP2 Antibodies for Specificity in Immunohistochemistry and Western Blotting"*
**作者**：Lee S, et al. (2022)
**摘要**：本研究系统评估了多种市售UCP2抗体的特异性，通过对比UCP2敲除细胞系及组织样本的WB和IHC结果，确认了抗体#AB123和#AB456在检测人/小鼠UCP2时的可靠性，为后续研究提供方法学参考。
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如需真实文献，建议通过PubMed、Google Scholar等平台检索关键词“UCP2 antibody validation”或“UCP2 immunohistochemistry”，并筛选近年高引文章。
Uncoupling protein 2 (UCP2) is a mitochondrial inner membrane protein belonging to the anion carrier family, primarily involved in regulating energy metabolism, oxidative stress, and cellular homeostasis. It uncouples oxidative phosphorylation from ATP production, dissipating the proton gradient to generate heat, thereby influencing metabolic efficiency and reactive oxygen species (ROS) modulation. UCP2 is expressed in various tissues, including the brain, pancreas, adipose tissue, and immune cells, and has been implicated in metabolic disorders, cancer, neurodegenerative diseases, and aging.
UCP2 antibodies are essential tools for detecting and quantifying UCP2 expression in research. They enable the study of UCP2's role in metabolic pathways, insulin secretion, inflammation, and cell survival. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specificity and validation are critical, as UCP2 shares structural homology with other UCP family members (e.g., UCP1. UCP3). High-quality antibodies must distinguish UCP2 from these isoforms and demonstrate minimal cross-reactivity.
Recent studies highlight UCP2's dual role in health and disease, where its upregulation or inhibition can either protect or exacerbate pathologies depending on context. Reliable UCP2 antibodies thus support advancing therapeutic research, particularly in metabolic syndrome, cancer metabolism, and neuroprotection. Researchers prioritize antibodies validated across multiple applications and species to ensure reproducibility in diverse experimental models.
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