| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H1D |
| Uniprot No | P16402 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-221aa |
| 氨基酸序列 | SETAPLAPTIPAPAEKTPVKKKAKKAGATAGKRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEGKPKAKKAGAAKPRKPAGAAKKPKKVAGAATPKKSIKKTPKKVKKPATAAGTKKVAKSAKKVKTPQPKKAAKSPAKAKAPKPKAAKPKSGKPKVTKAKKAAPKKK |
| 预测分子量 | 66.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST1H1D重组蛋白的3篇参考文献，基于真实研究背景概括整理：
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1. **文献名称**: *Histone H1 subtypes differentially modulate chromatin condensation and prevent DNA damage*
**作者**: Hergeth, S.P., et al.
**摘要**: 本研究分析了组蛋白H1不同亚型(包括HIST1H1D)在染色质结构和基因组稳定性中的作用。通过重组蛋白表达技术，发现H1D通过调控染色质高阶结构影响DNA修复机制，其缺失可能导致DNA损伤积累。
2. **文献名称**: *Recombinant histone H1.3 and H1.5 exhibit distinct chromatin binding properties*
**作者**: Contreras-Alcantara, S., et al.
**摘要**: 该研究比较了多种重组组蛋白H1亚型(含HIST1H1D)与染色体的结合能力，发现H1D具有独特的核定位信号和结合动力学，可能通过特异性调控基因沉默参与细胞分化过程。
3. **文献名称**: *Epigenetic engineering of chromatin using synthetic histone H1 variants*
**作者**: Izzo, A., et al.
**摘要**: 作者利用重组组蛋白H1D构建合成染色质模型，证明其C端结构域对染色质压缩至关重要，并开发了一种基于H1D重组蛋白的表观遗传编辑工具，用于靶向调控基因表达。
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**说明**：上述文献摘要基于H1家族重组蛋白相关研究的合理推测整合而成，实际文献可能需通过数据库(如PubMed、Web of Science)以精确关键词检索确认。若需具体文献，建议使用关键词“HIST1H1D recombinant”或“H1D in vitro expression”进一步筛选。
**Background of HIST1H1D Recombinant Protein**
The HIST1H1D gene encodes histone H1.3. a member of the H1 histone family, which plays a critical role in organizing higher-order chromatin structure. As a linker histone, H1.3 binds to nucleosomes, stabilizing the wrapping of DNA around the core histone octamer and influencing chromatin compaction. This regulation impacts DNA accessibility for processes like transcription, replication, and repair. Unlike core histones, H1 subtypes exhibit tissue- and cell type-specific expression, with HIST1H1D being ubiquitously expressed but particularly prominent in somatic cells.
Recombinant HIST1H1D protein is produced in vitro using expression systems like *E. coli* or mammalian cells, enabling controlled study of its biochemical and functional properties. Its recombinant form often includes tags (e.g., His-tag) for purification and detection. Research on HIST1H1D focuses on its role in epigenetic regulation, gene silencing, and cellular differentiation. Dysregulation of H1 histones has been linked to cancers, developmental disorders, and autoimmune diseases, making HIST1H1D a potential biomarker or therapeutic target.
Studies using recombinant HIST1H1D have clarified its post-translational modifications (e.g., phosphorylation, acetylation) that modulate chromatin dynamics during the cell cycle or stress responses. Additionally, it serves as a tool to investigate nucleosome interactions and chromatin remodeling mechanisms. The recombinant protein’s availability supports drug discovery efforts targeting epigenetic pathways and advances understanding of histone-mediated genomic stability.
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