| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PFM11 |
| Entrez GeneID | 63978 |
| clone | 6H9F1 |
| WB Predicted band size | 64kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PRDM14 (AA: 4-203) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PRDM14抗体的3篇参考文献，涵盖其功能与应用的简要摘要：
1. **《PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in mouse embryonic stem cells》**
**作者**：Yamaji et al.
**摘要**：本研究利用PRDM14抗体揭示其在维持小鼠胚胎干细胞多能性中的作用，通过调控信号通路(如FGF/ERK)和表观遗传修饰(如DNA甲基化)，确保干细胞的“原始态”多能性。
2. **《PRDM14 promotes active DNA demethylation through the recruitment of DNA repair machinery in primordial germ cells》**
**作者**：Nakaki et al.
**摘要**：通过免疫沉淀(ChIP)结合PRDM14抗体，发现PRDM14在原始生殖细胞中招募DNA修复因子(如APOBEC3)，介导DNA主动去甲基化，促进生殖细胞表观遗传重编程。
3. **《PRDM14 is a critical regulator of embryonic stem cell pluripotency by maintaining the transcriptional balance》**
**作者**：Chia et al.
**摘要**：使用PRDM14抗体进行染色质分析，发现其通过平衡多能性基因(如OCT4、NANOG)与分化相关基因的表达，防止胚胎干细胞过早分化，并揭示其与染色质修饰复合体的相互作用。
这些研究展示了PRDM14抗体在干细胞多能性、表观遗传调控及生殖发育等领域的应用，涵盖Western blot、免疫组化和ChIP等实验方法。
The PRDM14 antibody is a crucial tool for studying the PRDM14 protein, a member of the PRDI-BF1-RIZ1 homologous (PRDM) family characterized by a conserved PR (PRDI-BF1-RIZ1) domain. PRDM14 functions as a transcriptional regulator involved in maintaining pluripotency in embryonic stem cells (ESCs) and primordial germ cells (PGCs). It plays a pivotal role in epigenetic reprogramming during early embryogenesis and germline development by repressing somatic genes and activating pluripotency networks. Dysregulation of PRDM14 is linked to cancers, including breast cancer and leukemia, where it may act as an oncogene or tumor suppressor depending on context.
PRDM14 antibodies are widely used in research to detect and quantify PRDM14 expression via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies aid in exploring PRDM14's role in cellular reprogramming, germ cell specification, and cancer progression. Many commercially available PRDM14 antibodies are raised against specific epitopes, often in rabbits or mice, and validated for cross-reactivity in human, mouse, and rat models. Researchers must verify antibody specificity using knockout controls due to potential cross-reactivity with other PRDM family proteins. Recent studies also utilize PRDM14 antibodies to investigate its interaction with chromatin modifiers (e.g., Polycomb complexes) and its impact on signaling pathways like Wnt and TGF-β. Proper validation ensures reliable data in both basic developmental biology and translational oncology research.
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