| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2 |
| Entrez GeneID | 5606 |
| clone | 2E12D11 |
| WB Predicted band size | 39.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MAP2K3 (AA: 1-138) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是基于模拟生成的关于MAP2K3抗体的参考文献示例(请注意，以下内容为虚构示例，仅供格式参考)：
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1. **文献名称**: *MAP2K3 regulates inflammatory response via p38 activation in macrophages*
**作者**: Zhang L, et al.
**摘要**: 本研究利用MAP2K3特异性抗体通过Western blot和免疫沉淀技术，揭示了MAP2K3在巨噬细胞中通过磷酸化p38 MAPK调控炎症因子TNF-α和IL-6分泌的机制。抗体验证显示其在敲除细胞系中无交叉反应。
2. **文献名称**: *Expression profiling of MAP2K3 in hepatocellular carcinoma using immunohistochemistry*
**作者**: Tanaka K, et al.
**摘要**: 通过免疫组化分析肝癌组织中MAP2K3蛋白表达水平，发现其高表达与患者预后不良相关。研究中采用商业化MAP2K3抗体(货号AB123)，并进行了抗原修复优化以提高特异性。
3. **文献名称**: *MAP2K3 inhibition attenuates cardiac fibrosis in a mouse model*
**作者**: Smith J, et al.
**摘要**: 在小鼠心脏纤维化模型中，使用MAP2K3抗体检测到该激酶在心肌成纤维细胞中的激活状态。研究发现抑制MAP2K3可降低胶原沉积，抗体在Western blot中识别到约45 kDa的目标条带。
4. **文献名称**: *Development of a phospho-specific MAP2K3 antibody for signaling studies*
**作者**: Chen R, et al.
**摘要**: 本文报道了一种针对MAP2K3磷酸化位点(Ser189)的新型抗体的开发与验证。该抗体成功应用于流式细胞术和免疫荧光，证实其在氧化应激条件下MAP2K3的激活动力学。
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**备注**：以上文献为示例，实际引用需通过PubMed、Web of Science等数据库检索真实研究。建议使用关键词“MAP2K3 antibody”、“MAP2K3 + [疾病/通路]”进行精准查询。
The MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3) antibody is a tool used to detect and study the MAP2K3 protein, a dual-specificity kinase within the MAPK signaling pathway. MAP2K3. also known as MEK3. plays a critical role in mediating cellular responses to stress, inflammation, and differentiation. It functions downstream of various stimuli (e.g., cytokines, osmotic stress) by phosphorylating and activating p38 MAP kinase, which regulates transcription factors and cellular processes like apoptosis, immune responses, and cell cycle progression. Dysregulation of MAP2K3/p38 signaling is implicated in diseases such as cancer, neurodegenerative disorders, and autoimmune conditions.
MAP2K3 antibodies are widely used in research to investigate protein expression, localization, and activation states via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specific antibodies targeting phosphorylated MAP2K3 (e.g., at Ser189/Thr193) help assess its enzymatic activity. Validation of these antibodies typically includes testing in knockout models or siRNA-treated cells to confirm specificity. Researchers also employ them to explore MAP2K3's role in signaling crosstalk, drug response, and pathway modulation in disease models. Reliable MAP2K3 antibodies are essential for elucidating its biological functions and therapeutic potential in targeting MAPK-related pathologies.
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