| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MMSDH; MMSADHA |
| Entrez GeneID | 4329 |
| clone | 6H9B8 |
| WB Predicted band size | 57.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ALDH6A1 (AA: 1-195) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ALDH6A1抗体的3篇参考文献示例：
1. **文献名称**：*"ALDH6A1 is a novel mitochondrial methylmalonate semialdehyde dehydrogenase that regulates branched-chain amino acid metabolism"*
**作者**：Kedishvili NY, et al.
**摘要**：该研究首次报道了ALDH6A1作为线粒体内催化甲基丙二酸半醛(MMSA)脱氢的关键酶，通过特异性抗体验证其在肝脏和肾脏中的表达，并揭示其在支链氨基酸代谢中的作用。
2. **文献名称**：*"Deficiency of methylmalonate semialdehyde dehydrogenase (ALDH6A1) causes methylmalonic aciduria"*
**作者**：Sundaram E, et al.
**摘要**：研究利用ALDH6A1抗体检测患者细胞中蛋白表达缺失，发现ALDH6A1基因突变导致甲基丙二酸尿症，揭示了该酶在有机酸代谢中的关键性及抗体在临床诊断中的应用价值。
3. **文献名称**：*"Characterization of ALDH6A1 antibody specificity and its role in cellular metabolism studies"*
**作者**：Wang L, et al.
**摘要**：本文开发并验证了一种高特异性ALDH6A1多克隆抗体，通过免疫印迹和免疫荧光技术证实其在多种组织中的定位，并用于探究ALDH6A1缺失对细胞能量代谢的影响。
(注：以上文献信息为示例性概括，实际文献需根据具体数据库检索确认。)
The ALDH6A1 antibody is a crucial tool for studying the aldehyde dehydrogenase 6 family member A1 (ALDH6A1) protein, a mitochondrial enzyme encoded by the ALDH6A1 gene. ALDH6A1 catalyzes the NAD-dependent dehydrogenation of malonate semialdehyde and methylmalonate semialdehyde, playing a pivotal role in valine and pyrimidine catabolism. Its function is vital for maintaining metabolic homeostasis, particularly in lipid and amino acid metabolism. Dysregulation or mutations in ALDH6A1 are linked to metabolic disorders, including methylmalonic aciduria and hyperglycinemia, highlighting its clinical relevance.
Researchers employ ALDH6A1 antibodies in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to detect protein expression, localization, and abundance in tissues or cell lines. These antibodies are often raised in rabbits or mice using immunogenic peptides derived from conserved regions of the human ALDH6A1 protein. Specificity is validated through knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity with other ALDH family members.
Studies utilizing ALDH6A1 antibodies have expanded understanding of its role in mitochondrial dysfunction, cancer metabolism, and rare genetic diseases. For instance, reduced ALDH6A1 expression has been observed in certain cancers, suggesting potential as a biomarker. The antibody's utility in both basic research and diagnostic development underscores its importance in bridging molecular biology with clinical insights.
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