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Human α2 macroglobulin,α2 MG ELISA Kit

  • 中文名： 人α2巨球蛋白(α2-MG)ELISA Kit
货号: IPD-E08959h
Price： ￥2500
数量：
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产品详情

  • 产品描述：人α2巨球蛋白(α2-MG)酶联免疫试剂盒（CSB-E08959h）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的A2M含量。A2M（α2-巨球蛋白）是一种血浆中含量丰富的大分子糖蛋白，具有广谱蛋白酶抑制活性，参与宿主防御机制。其研究机制涉及与多种细胞因子、生长因子和激素结合，调节体内平衡，发挥保护作用。在奶牛乳腺炎抗性研究中，A2M基因的突变可增强其表达活性，提高奶牛抗病力。试剂盒检测范围为1.25 μg/mL-20 μg/mL，灵敏度为1.25 μg/mL。适用于科研领域中对α2 - MG表达水平的研究，例如炎症相关疾病机制探索、免疫调控分析或组织损伤修复模型中的生物标志物检测，支持多种生物样本的灵活检测需求，为体外实验提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：A2m ELISA Kit; A2MG_HUMAN ELISA Kit; Alpha 2 M ELISA Kit; Alpha 2M ELISA Kit; Alpha-2-M ELISA Kit; Alpha-2-macroglobulin ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 ELISA Kit; CPAMD5 ELISA Kit; DKFZp779B086 ELISA Kit; FWP007 ELISA Kit; S863 7 ELISA Kit

  • 缩写：A2M

  • Uniprot No.：P01023

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, tissue homogenates

  • 检测范围：1.25 μg/mL-20 μg/mL

  • 灵敏度：1.25 μg/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Immunology

  • 测定原理：quantitative

  • 测定方法：Competitive

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human α2 MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:100Average %87
    Range %82-93
    1:200Average %94
    Range %89-99
    1:400Average %95
    Range %89-99
    1:800Average %92
    Range %87-96

  • 回收率：

    The recovery of human α2 MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9285-98
    EDTA plasma (n=4)9692-101














    标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average  200.1850.1870.186  100.3600.3650.363  50.6350.6420.639  2.51.1631.1721.168  1.251.9952.0132.004  02.8492.9242.887  






  • 货期：3-5 working days


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