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Recombinant Human slpA protein

  • 中文名: 外膜蛋白SlpA(slpA)重组蛋白
  • 别    名: slpA;Outer membrane protein SlpA
货号: PA2000-4567
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点slpA
Uniprot No P32053
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-413aa
氨基酸序列MARKTKPLTD TEIKAAKPKD ADYQLYDGDG LTLLIKSSGS KLWQFRYYRP LTKQRTKQSF GAYPAVSLSD ARKLRAESKV LLAKDIDPQE HQKEQVRNSQ EAKTNTFLLV AERWWNVKKT SVTEDYADDI WRSLERDIFP AIGDISITEI KAHTLVKAVQ PVQARGALET VRRLCQRINE VMIYAQNTGL IDAVPSVNIG KAFEKPQKKN MPSIRPDQLP QLMHTMRTAS ISMSTRCLFM WQLLTITRPA EAAEARWDEI DFNASEWKIP AARMKMNRDH TVPLSDGALA ILEMMKPLSG GREFIFPSRI KPNQPMNSQT VNAALKRAGL GGVLVSHGLR SIASTALNEE GFPPDVIEAA LAHVDKNEVR RAYNRSDYLE QRRPMMQWWA DLVKAADSGS IVLTHLSKIR LVG
预测分子量46,6 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇关于slpA重组蛋白的参考文献摘要示例(注:文献信息为模拟概括,实际引用请核对原文):

1. **文献名称**: *Cloning and expression of the slpA gene in Lactobacillus acidophilus*

**作者**: Smith J, et al.

**摘要**: 本研究克隆了L. acidophilus的slpA基因并在大肠杆菌中成功表达,证实重组SlpA蛋白具有促进肠道细胞粘附的功能,为益生菌表面蛋白应用提供基础。

2. **文献名称**: *Immunomodulatory effects of recombinant SlpA protein in murine model*

**作者**: Wang L, et al.

**摘要**: 通过纯化重组SlpA蛋白并注射小鼠,发现其可激活巨噬细胞并增强Th1型免疫应答,提示SlpA在疫苗佐剂开发中的潜在价值。

3. **文献名称**: *Structural characterization of S-layer protein SlpA by cryo-EM*

**作者**: Tanaka K, et al.

**摘要**: 利用冷冻电镜解析了重组SlpA蛋白的纳米级晶格结构,揭示了其自组装机制及pH依赖性构象变化,为工程化生物材料设计提供结构依据。

注:以上内容基于典型研究方向模拟,实际文献需通过PubMed/Google Scholar以"slpA recombinant protein"为关键词检索获取。

背景信息

**Background of S-layer Protein A (SlpA) Recombinant Protein**

S-layer protein A (SlpA) is a key structural component of the surface layer (S-layer) found in many bacteria, particularly within the *Lactobacillus* genus, such as *Lactobacillus acidophilus*. The S-layer is a paracrystalline array of proteins that coats the cell surface, serving as a protective barrier and mediating interactions between the bacterium and its environment. SlpA plays a critical role in adhesion to host tissues, immune modulation, and resistance to environmental stressors, making it a focal point in probiotic research and therapeutic development.

Recombinant SlpA is produced through genetic engineering, often using heterologous expression systems like *E. coli* or yeast. This approach allows large-scale production of purified SlpA for functional studies. Structurally, SlpA typically contains conserved domains, such as S-layer homology (SLH) domains, which anchor the protein to the cell wall, and variable regions that dictate host-specific interactions. Its ability to self-assemble into ordered lattices has sparked interest in nanotechnology and vaccine design.

Research highlights SlpA’s role in probiotic-host interactions, including immune system activation via Toll-like receptors (TLRs) and competitive exclusion of pathogens. Recombinant SlpA is also explored as a vaccine adjuvant or drug delivery vehicle due to its biocompatibility and targeting potential. Additionally, studies on SlpA variants shed light on evolutionary adaptations of probiotic strains to specific niches, such as the gastrointestinal tract.

Despite progress, challenges remain in understanding its precise molecular mechanisms and optimizing recombinant production for clinical applications. Ongoing work aims to harness SlpA’s multifunctional properties for innovative therapies, diagnostics, and bioengineered materials.

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