| 纯度 | >90%SDS-PAGE. |
| 种属 | Drosophila |
| 靶点 | Ect4 |
| Uniprot No | Q6IDD9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 370-678aa |
| 氨基酸序列 | KSQYLEKINEVIRRAWAVPTHGHELGYSLCNSLRQSGGLDLLMKNCVKPDLQFSSAQLLEQCLTTENRKHVVDNGLDKVVNVACVCTKNSNMEHSRVGTGILEHLFKHSEGTCSDVIRLGGLDAVLFECRTSDLETLRHCASALANLSLYGGAENQEEMILRKVPMWLFPLAFHNDDNIKYYACLAIAVLVANKEIEAEVLKSGCLDLVEPFVTSHDPSAFARSNLAHAHGQSKHWLKRLVPVLSSNREEARNLAAFHFCMEAGIKREQGNTDIFREINAIEALKNVASCPNAIASKFAAQALRLIGET |
| 预测分子量 | 41.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ECT4重组蛋白的3篇参考文献示例(注：以下文献为虚构示例，仅供参考格式)：
1. **文献名称**：*"Heterologous Expression and Functional Characterization of Recombinant ECT4 Protein in Yeast"*
**作者**：Chen, J. et al.
**摘要**：研究报道了ECT4基因在酵母系统中的重组表达，通过亲和层析纯化获得高纯度蛋白，并证实其在细胞膜运输中的ATP酶活性。
2. **文献名称**：*"Structural Insights into ECT4 Recombinant Protein and Its Role in mRNA Export"*
**作者**：Kim, S. & Park, H.
**摘要**：利用X射线晶体学解析了重组ECT4蛋白的三维结构，揭示了其与RNA结合的结构域，并验证其通过核孔复合体参与mRNA转运的功能。
3. **文献名称**：*"Optimization of ECT4 Recombinant Protein Production in Insect Cells for Therapeutic Screening"*
**作者**：Gomez, R. et al.
**摘要**：优化了昆虫细胞表达系统中ECT4重组蛋白的产量，评估其作为药物靶点的潜力，并筛选了小分子抑制剂。
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**注意事项**：
- 实际文献需通过PubMed、Google Scholar等平台以“ECT4 recombinant protein”“ECT4 gene function”等关键词检索。若结果有限，可尝试扩展搜索词(如物种名+ECT4)。
- ECT4可能为特定模式生物(如果蝇、拟南芥)中的基因，需结合研究领域调整检索策略。
**Background of Ect4 Recombinant Protein**
Ect4. originally identified in *Drosophila melanogaster*, is a member of the ECT2/TOB family of proteins, which are evolutionarily conserved across eukaryotes. These proteins are characterized by their roles in regulating mRNA stability, translation, and localization, often via interactions with RNA-binding proteins or components of the cytoplasmic mRNA processing machinery. Ect4 specifically has been implicated in modulating cell polarity, epithelial morphogenesis, and developmental processes by influencing mRNA transport and localized translation within cellular compartments.
The recombinant Ect4 protein is engineered through molecular cloning, where the *Ect4* gene is expressed in heterologous systems like *E. coli* or insect cells. This allows large-scale production of the protein for functional studies. Recombinant Ect4 typically retains key domains, such as its conserved N-terminal region and putative RNA-binding motifs, enabling researchers to dissect its biochemical interactions and structural properties *in vitro*.
Studies using recombinant Ect4 have advanced understanding of its role in mRNA regulation and its crosstalk with signaling pathways, such as the Wnt or Hippo pathways, which are critical in development and cancer. Additionally, Ect4 homologs in mammals (e.g., human TOB1/2) are linked to tumor suppression and immune regulation, highlighting its broader biomedical relevance. The availability of recombinant Ect4 facilitates drug screening, structural analysis, and mechanistic studies, offering potential insights into therapies targeting mRNA dysregulation in diseases like cancer or neurodegeneration.
In summary, Ect4 recombinant protein serves as a vital tool for exploring post-transcriptional gene regulation and its impact on cellular dynamics, development, and disease.
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