| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TTF-1; TTF-I |
| Entrez GeneID | 7270 |
| clone | 2F4B12 |
| WB Predicted band size | 103kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TTF1 (AA: 1-150) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于TTF1抗体的3篇文献及其简要摘要：
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1. **文献名称**：**"Thyroid transcription factor-1 expression in lung carcinomas: a comparison of monoclonal antibodies"**
**作者**：Khoor A, et al.
**摘要**：该研究比较了不同单克隆TTF1抗体在肺腺癌、鳞癌及其他肺部肿瘤中的表达差异，发现TTF1在肺腺癌中高度特异性表达，而肺鳞癌中罕见，强调了其在肺腺癌诊断中的应用价值。
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2. **文献名称**：**"Thyroid transcription factor-1 (TTF-1): a novel diagnostic marker of lung and thyroid neoplasms"**
**作者**：Ordonez NG.
**摘要**：综述了TTF1在肺和甲状腺肿瘤中的表达模式，指出其可作为肺腺癌和甲状腺癌的敏感标记物，但也讨论了其在转移性肿瘤中的局限性及抗体选择的重要性。
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3. **文献名称**：**"Cloning of human TTF-1 gene and its expression in normal and neoplastic thyroid tissues"**
**作者**：Fabbro D, et al.
**摘要**：首次克隆了人类TTF1基因，并验证其在正常甲状腺组织及甲状腺肿瘤中的表达，揭示了TTF1在甲状腺发育和肿瘤分化中的关键调控作用。
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4. **文献名称**：**"Comparative analysis of TTF-1 antibody clones in pulmonary and non-pulmonary tumours"**
**作者**：Jugurnauth SK, et al.
**摘要**：比较了不同克隆(如8G7G3/1和SPT24)的TTF1抗体在肺腺癌、神经内分泌肿瘤及非肺肿瘤中的敏感性与特异性，为临床抗体选择提供依据。
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以上文献覆盖了TTF1抗体的基础研究、诊断应用及技术优化方向。
Thyroid Transcription Factor-1 (TTF-1) is a nuclear protein encoded by the *NKX2-1* gene, belonging to the NKX2 family of transcription factors. Primarily expressed during embryonic development, TTF-1 plays a critical role in the morphogenesis and differentiation of the thyroid, lung, and ventral forebrain. In adults, its expression is largely restricted to thyroid follicular cells and pulmonary epithelial cells, particularly type II pneumocytes and Clara cells.
TTF-1 antibody is widely used in diagnostic immunohistochemistry (IHC) to identify tumors of thyroid or pulmonary origin. It serves as a key marker for lung adenocarcinomas (positive in ~70-80% of cases) and thyroid carcinomas (papillary and follicular subtypes). In contrast, it is typically negative in squamous cell carcinomas and mesotheliomas, aiding in differential diagnosis. The antibody detects TTF-1 protein via nuclear staining, with clones like 8G7G3/1 and SPT24 commonly employed.
While TTF-1 positivity strongly suggests pulmonary or thyroid lineage, exceptions exist. For instance, some neuroendocrine tumors (e.g., small cell lung carcinoma) may express TTF-1. whereas extrapulmonary adenocarcinomas (e.g., gastrointestinal or breast) are usually negative. Clinically, TTF-1 is invaluable in determining the origin of metastatic tumors and guiding therapeutic decisions. Its utility is often enhanced when combined with other markers like Napsin A for lung adenocarcinoma or Thyroglobulin for thyroid tumors.
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