纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | AP3B2 |
Uniprot No | Q13367 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 973-1078aa |
氨基酸序列 | APVFMSENEFKKEQGKLMGMNEITEKLMLPDTCRSDHIVVQKVTATANLGRVPCGTSDEYRFAGRTLTGGSLVLLTLDARPAGAAQLTVNSEKMVIGTMLVKDVIQ |
预测分子量 | 19.0 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP3B2重组蛋白的3篇参考文献及其摘要概括:
1. **"Recombinant AP3B2 regulates synaptic vesicle protein sorting in neuronal cells"**
- **作者**: Smith J. et al.
- **摘要**: 研究利用重组AP3B2蛋白在神经元细胞中验证其功能,发现其通过调控突触小泡相关蛋白的分选参与神经递质释放,为神经退行性疾病机制提供新视角。
2. **"Expression and functional characterization of AP3B2 in Alzheimer's disease models"**
- **作者**: Chen L. & Wang H.
- **摘要**: 通过重组AP3B2蛋白体外表达,揭示其在阿尔茨海默病模型中与β-淀粉样蛋白的异常相互作用,可能影响神经元内运输通路并加剧病理进展。
3. **"AP3B2 recombinant protein as a potential biomarker for neurodevelopmental disorders"**
- **作者**: Kim S. et al.
- **摘要**: 研究开发了高纯度重组AP3B2蛋白,并验证其作为神经发育障碍(如自闭症)患者血清中潜在诊断标记物的可能性,为临床检测提供新工具。
注:以上文献信息为模拟概括,实际文献需通过PubMed、Google Scholar等平台检索确认。建议结合具体研究方向补充更精准的参考文献。
**Background of AP3B2 Recombinant Protein**
The AP3B2 (Adaptor-Related Protein Complex 3 Beta 2 Subunit) is a critical component of the heterotetrameric AP-3 complex, which mediates intracellular protein sorting and vesicle trafficking. The AP-3 complex, comprising β, δ, μ, and σ subunits, facilitates cargo selection and transport from endosomes to lysosomes or lysosome-related organelles (LROs). AP3B2. a neuron-specific isoform of the β subunit, diverges from the ubiquitously expressed AP3B1. suggesting specialized roles in neural development and function.
AP3B2 is prominently expressed in the brain, particularly in synaptic regions, where it regulates neurotransmitter release and synaptic vesicle formation. Studies link AP3B2 to the biogenesis of synaptic vesicles and the trafficking of vesicular transporters, such as VGLUT1. impacting neuronal communication. Dysregulation of AP3B2 has been associated with neurodevelopmental disorders, including autism spectrum disorders and intellectual disabilities, highlighting its importance in maintaining neural homeostasis.
Recombinant AP3B2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce a purified, functional form for *in vitro* studies. This protein often includes affinity tags (e.g., His-tag) for simplified purification and detection. Researchers utilize recombinant AP3B2 to investigate its interactions with other AP-3 subunits, cargo recognition mechanisms, and involvement in vesicular trafficking pathways. Additionally, it aids in exploring pathological mechanisms caused by AP3B2 mutations and screening therapeutic agents targeting AP-3-related neurological disorders.
Overall, AP3B2 recombinant protein serves as a vital tool for dissecting the molecular basis of neuronal vesicle dynamics and developing insights into neurodevelopmental diseases. Its study bridges gaps between cellular trafficking mechanisms and neurological health, offering potential avenues for therapeutic intervention.
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