| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDFY1 |
| Uniprot No | Q8IWB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-410aa |
| 氨基酸序列 | MAAEIHSRPQSSRPVLLSKIEGHQDAVTAALLIPKEDGVITASEDRTIRVWLKRDSGQYWPSIYHTMASPCSAMAYHHDSRRIFVGQDNGAVMEFHVSEDFNKMNFIKTYPAHQNRVSAIIFSLATEWVISTGHDKCVSWMCTRSGNMLGRHFFTSWASCLQYDFDTQYAFVGDYSGQITLLKLEQNTCSVITTLKGHEGSVACLWWDPIQRLLFSGASDNSIIMWDIGGRKGRTLLLQGHHDKVQSLCYLQLTRQLVSCSSDGGIAVWNMDVSREEAPQWLESDSCQKCEQPFFWNIKQMWDTKTLGLRQHHCRKCGQAVCGKCSSKRSSYPVMGFEFQVRVCDSCYDSIKDEDRTSLATFHEGKHNISHMSMDIARGLMVTCGTDRIVKIWDMTPVVGCSLATGFSPH |
| 预测分子量 | 53.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WDFY1重组蛋白的3篇参考文献示例(内容基于假设性研究，实际文献需通过数据库验证)：
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1. **文献名称**: *WDFY1 regulates autophagic activity through interactions with LC3 family proteins*
**作者**: Smith J, et al. (2020)
**摘要**: 本研究利用重组WDFY1蛋白在原核系统中表达并纯化，通过体外pull-down实验证实其直接结合LC3蛋白，揭示了WDFY1作为自噬接头蛋白的分子机制。
2. **文献名称**: *Structural characterization of WDFY1 recombinant protein and its role in vesicular trafficking*
**作者**: Johnson R, et al. (2018)
**摘要**: 通过昆虫细胞系统表达重组WDFY1蛋白，采用X射线晶体学解析其WD40结构域的三维结构，发现该结构域对细胞内囊泡运输中的蛋白相互作用至关重要。
3. **文献名称**: *High-throughput screening platform identifies WDFY1 as a modulator of mTOR signaling*
**作者**: Lee H, et al. (2021)
**摘要**: 构建重组WDFY1蛋白的真核表达系统，结合药物筛选技术，发现其通过调控mTORC1复合体的活性影响细胞生长，为相关疾病治疗提供潜在靶点。
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**注意**：以上为模拟数据，实际文献需通过PubMed、Google Scholar等平台检索关键词“WDFY1 recombinant protein”或“WDFY1 protein expression”获取。若需具体文献协助，请提供更详细的研究方向或背景。
WDFY1 (WD repeat and FYVE domain-containing protein 1) is a multidomain protein encoded by the *WDFY1* gene, belonging to the WDFY family. It is characterized by its conserved structural motifs: WD40 repeats and a FYVE domain. The WD40 repeats facilitate protein-protein interactions, while the FYVE domain binds phosphatidylinositol 3-phosphate (PI3P), linking WDFY1 to membrane trafficking and signaling processes. Although its exact biological role remains under investigation, WDFY1 is implicated in autophagy, immune regulation, and intracellular vesicle transport. Studies suggest it acts as a scaffold protein, coordinating molecular complexes involved in autophagosome formation, particularly in selective autophagy pathways like xenophagy (clearance of intracellular pathogens) and mitophagy (removal of damaged mitochondria).
Recombinant WDFY1 protein, produced through heterologous expression systems (e.g., *E. coli*, mammalian cells), serves as a critical tool for functional studies. Researchers utilize it to dissect molecular interactions, map binding partners, and explore its role in autophagy-related signaling cascades, such as mTOR or ULK1 pathways. Dysregulation of WDFY1 has been associated with diseases, including cancers and neurodegenerative disorders, where autophagy defects are prominent. For instance, reduced WDFY1 expression correlates with impaired antigen presentation in immune cells, hinting at its involvement in adaptive immunity.
Despite progress, many aspects of WDFY1 remain enigmatic. Its tissue-specific functions, post-translational modifications, and crosstalk with other autophagy regulators (e.g., ATG proteins) are active research areas. Recombinant WDFY1 also holds potential for therapeutic development, particularly in modulating autophagy to treat infection, cancer, or metabolic diseases. Further structural and mechanistic studies are needed to clarify its precise contributions to cellular homeostasis and pathology.
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