| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TDT |
| Entrez GeneID | 1791 |
| clone | 4B10A6 |
| WB Predicted band size | 58.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human DNTT (AA: 52-192) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于DNTT(Terminal Deoxynucleotidyl Transferase，TdT)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: **"Terminal deoxynucleotidyl transferase in human leukemia"**
**作者**: Bollum FJ, Brown M
**摘要**: 该研究首次系统分析了DNTT(TdT)在白血病中的表达特征，验证了其作为急性淋巴细胞白血病(ALL)特异性标记物的潜力。作者利用兔源多克隆抗体进行免疫荧光实验，发现TdT在大多数ALL患者原始细胞中高表达，而在正常成熟淋巴细胞中缺失，为白血病分型提供了重要依据。
2. **文献名称**: **"Monoclonal antibody specific for terminal deoxynucleotidyl transferase: Characterization and use in leukemia diagnosis"**
**作者**: Kung PC, Long JC, McCaffrey RP, et al.
**摘要**: 研究团队开发了一种鼠源单克隆抗TdT抗体，并通过流式细胞术和免疫组化验证其特异性。该抗体能精准识别胸腺细胞和白血病母细胞中的TdT蛋白，显著提高了急性白血病(尤其是T-ALL)的诊断准确性，并推动了临床免疫分型技术的标准化。
3. **文献名称**: **"Terminal deoxynucleotidyl transferase expression in immature lymphocytes: Insights into lymphoid differentiation"**
**作者**: Gregoire KE, Goldschneider I
**摘要**: 文章探讨了TdT在淋巴细胞早期发育中的功能，利用抗TdT抗体标记小鼠骨髓及胸腺细胞，证实TdT通过介导V(D)J重排的多样性促进免疫受体基因重组。研究还发现TdT表达水平与淋巴细胞分化阶段密切相关，为免疫发育生物学提供了关键证据。
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以上文献涵盖了DNTT抗体的临床应用(白血病诊断)、抗体开发及分子机制研究领域。如需更多文献或具体细节，可进一步检索PubMed或Web of Science数据库。
Terminal deoxynucleotidyl transferase (TdT), encoded by the DNTT gene, is a specialized DNA polymerase expressed during early lymphocyte development. Unlike other DNA polymerases, TdT catalyzes template-independent addition of nucleotides to DNA ends, a critical process in generating antigen receptor diversity through V(D)J recombination. It randomly incorporates nucleotides at junctional regions of immunoglobulin and T-cell receptor genes, creating unique antigen-binding sites. TdT is predominantly found in immature precursor B-cells and T-cells in bone marrow and thymus, with minimal expression in mature lymphocytes.
Anti-DNTT antibodies are valuable tools in research and diagnostics. In clinical pathology, TdT detection via antibodies helps identify acute lymphoblastic leukemias (ALL) and lymphoblastic lymphomas, as these malignancies often involve TdT-positive immature lymphoid cells. Commercially available monoclonal antibodies against TdT enable immunohistochemical or flow cytometry-based differentiation of ALL from other hematologic cancers. Researchers also use DNTT antibodies to study lymphocyte maturation defects and immune repertoire development. However, TdT expression isn't entirely leukemia-specific; rare cases of non-hematopoietic tumors may show aberrant TdT positivity. Optimized antibody validation remains essential given variable staining patterns across tissue types and fixation methods.
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