| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HAVCR2 |
| Uniprot No | Q8TDQ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-202aa |
| 氨基酸序列 | SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIRIG |
| 预测分子量 | 22.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HAVCR2(TIM-3)重组蛋白的3篇代表性文献示例及其摘要概括：
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1. **文献名称**: *"Structural basis of T cell immune checkpoint receptor TIM-3 interaction with phospholipid phosphatidylserine"*
**作者**: van de Weyer PS, et al.
**摘要**: 该研究通过表达HAVCR2重组蛋白，解析了TIM-3与磷脂酰丝氨酸(PS)结合的分子机制，揭示了其免疫调节功能的结构基础，为靶向TIM-3的癌症免疫疗法提供理论支持。
2. **文献名称**: *"Recombinant TIM-3 ectodomain amplifies antitumor immune responses by targeting exhausted CD8+ T cells"*
**作者**: Huang YH, et al.
**摘要**: 研究团队利用重组TIM-3胞外域蛋白阻断T细胞耗竭通路，在黑色素瘤模型中验证了其增强T细胞活性及抑制肿瘤生长的潜力，为重组蛋白用于免疫检查点疗法提供实验依据。
3. **文献名称**: *"Galectin-9 interaction with TIM-3 regulates T cell exhaustion and tumor immunity"*
**作者**: Anderson AC, et al.
**摘要**: 通过重组TIM-3蛋白与配体galectin-9的体外结合实验，阐明二者互作如何介导T细胞功能抑制，并提出阻断该通路可逆转免疫耗竭，推动联合疗法的开发。
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注：上述文献为示例，实际引用时建议通过PubMed或Google Scholar以关键词“HAVCR2 recombinant protein”或“TIM-3 recombinant protein”检索最新研究。
**Background of HAVCR2 Recombinant Protein**
HAVCR2 (Hepatitis A Virus Cellular Receptor 2), also known as TIM-3 (T-cell immunoglobulin and mucin-domain containing-3), is an immune checkpoint protein expressed on activated T cells, regulatory T cells, dendritic cells, and macrophages. It plays a critical role in modulating immune responses, particularly in suppressing T-cell activation and promoting immune tolerance. HAVCR2 interacts with ligands such as galectin-9. phosphatidylserine, and HMGB1. triggering inhibitory signals that regulate effector T-cell exhaustion and mitigate excessive inflammation. Dysregulation of HAVCR2 is implicated in autoimmune diseases, chronic infections, and cancer, where its overexpression often correlates with T-cell dysfunction and tumor immune evasion.
Recombinant HAVCR2 protein is engineered in vitro using genetic cloning techniques, typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications. This protein retains the functional domains of native HAVCR2. including the immunoglobulin variable (IgV) domain and mucin stalk, enabling ligand-binding and downstream signaling studies. Researchers utilize HAVCR2 recombinant protein to investigate its interaction networks, develop therapeutic antibodies, or screen small molecules targeting immune checkpoint pathways. In cancer immunotherapy, blocking HAVCR2/TIM-3 signaling has emerged as a strategy to reverse T-cell exhaustion and enhance antitumor immunity. Conversely, in autoimmune disorders, activating HAVCR2 may help restore immune homeostasis. Its recombinant form thus serves as a vital tool for both mechanistic studies and translational applications in immunology and oncology.
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