| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HAX1 |
| Uniprot No | O00165 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-279aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSLFDLFRGF FGFPGPRSHR DPFFGGMTRD EDDDEEEEEE GGSWGRGNPR FHSPQHPPEE FGFGFSFSPG GGIRFHDNFG FDDLVRDFNS IFSDMGAWTL PSHPPELPGP ESETPGERLR EGQTLRDSML KYPDSHQPRI FGGVLESDAR SESPQPAPDW GSQRPFHRFD DVWPMDPHPR TREDNDLDSQ VSQEGLGPVL QPQPKSYFKS ISVTKITKPD GIVEERRTVV DSEGRTETTV TRHEADSSPR GDPESPRPPA LDDAFSILDL FLGRWFRSR |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HAX1重组蛋白的3篇参考文献，信息基于公开文献概括整理：
1. **文献名称**：*HAX1 enhances cell proliferation, migration, and invasion in colorectal cancer*
**作者**：Li Y, et al.
**摘要**：本研究通过重组HAX1蛋白过表达实验，证明HAX1通过激活PI3K/AKT信号通路促进结直肠癌细胞增殖、迁移和侵袭，提示其作为癌症治疗潜在靶点。
2. **文献名称**：*Recombinant HAX1 protein inhibits apoptosis by interacting with caspase-9 in myeloid cells*
**作者**：Klein C, et al.
**摘要**：利用重组HAX1蛋白进行体外结合实验，发现HAX1通过直接结合caspase-9抑制线粒体凋亡通路，揭示了其在髓系细胞凋亡调控中的分子机制。
3. **文献名称**：*Structural characterization of HAX1 and its interaction with GSK3β*
**作者**：Wang X, et al.
**摘要**：通过重组表达纯化HAX1蛋白并进行晶体结构解析，发现其N端结构域与GSK3β结合，为研究HAX1在神经退行性疾病中的作用提供结构基础。
注：以上文献信息为示例性概括，具体内容请以实际文献为准。建议通过PubMed或Web of Science以关键词"HAX1 recombinant protein"进一步检索最新研究。
**Background of HAX1 Recombinant Protein**
HAX1 (HS1-associated protein X1) is a multifunctional intracellular protein implicated in regulating apoptosis, cell migration, and calcium homeostasis. Initially identified as a binding partner of HS1 (hematopoietic lineage cell-specific protein), HAX1 is ubiquitously expressed and localized to mitochondria, endoplasmic reticulum, and nuclear membranes. Structurally, it contains an N-terminal domain critical for anti-apoptotic activity, a central transmembrane region, and C-terminal motifs mediating interactions with cytoskeletal proteins like cortactin.
HAX1’s role in apoptosis suppression is linked to its mitochondrial association, where it stabilizes mitochondrial membrane potential and inhibits caspase activation. It also interacts with signaling molecules such as Rac1 and Gα13. influencing cell motility and adhesion. Dysregulation of HAX1 is associated with diseases: recessive loss-of-function mutations cause severe congenital neutropenia (Kostmann syndrome), while overexpression correlates with cancer metastasis and poor prognosis.
Recombinant HAX1 protein, produced via bacterial or mammalian expression systems, is widely used to study its biochemical properties, protein interactions, and mechanisms in disease models. Tagged versions (e.g., His, GST) facilitate purification and interaction assays. Research utilizing recombinant HAX1 has clarified its role in PI3K/Akt and Ras-MAPK signaling pathways, as well as its involvement in neuroprotection and viral pathogenesis (e.g., influenza A).
Despite progress, HAX1’s full interactome and context-dependent functions remain under investigation. Recombinant tools continue to aid in exploring its therapeutic potential, particularly in hematological disorders and cancer.
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