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Recombinant Human Rpl7 protein

  • 中文名: 大核糖体亚基蛋白uL30(Rpl7)重组蛋白
  • 别    名: Rpl7;Large ribosomal subunit protein uL30
货号: PA2000-4661
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点Rpl7
Uniprot No P18124
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-248aa
氨基酸序列MEGVEEKKKE VPAVPETLKK KRRNFAELKI KRLRKKFAQK MLRKARRKLI YEKAKHYHKE YRQMYRTEIR MARMARKAGN FYVPAEPKLA FVIRIRGING VSPKVRKVLQ LLRLRQIFNG TFVKLNKASI NMLRIVEPYI AWGYPNLKSV NELIYKRGYG KINKKRIALT DNALIARSLG KYGIICMEDL IHEIYTVGKR FKEANNFLWP FKLSSPRGGM KKKTTHFVEG GDAGNREDQI NRLIRRMN
预测分子量29,2 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇关于Rpl7重组蛋白的假设性参考文献示例(注:内容为虚构,仅作格式参考):

1. **《Recombinant Rpl7 Promotes Ribosome Assembly in Vitro》**

- 作者:Zhang, Y. et al.

- 摘要:本研究利用大肠杆菌表达系统成功纯化重组Rpl7蛋白,并通过体外核糖体重组实验证明其能有效促进核糖体大亚基的组装,揭示了Rpl7在核糖体生物合成中的分子机制。

2. **《Functional Analysis of Rpl7 in Zebrafish Development Using Recombinant Protein Rescue》**

- 作者:Chen, L. & Wang, H.

- 摘要:通过注射重组Rpl7蛋白至Rpl7基因敲除斑马鱼胚胎,发现其可部分恢复胚胎发育异常,表明Rpl7在脊椎动物早期发育中具有保守的调控功能。

3. **《Rpl7 Recombinant Protein Binds c-Myc mRNA and Modulates Translation Efficiency》**

- 作者:Kim, S. et al.

- 摘要:研究发现重组人源Rpl7蛋白可直接结合c-Myc mRNA的5'UTR区域,并通过改变核糖体结合活性调控其翻译效率,提示Rpl7在肿瘤发生中的潜在作用。

背景信息

**Background of Recombinant RPL7 Protein**

Ribosomal protein L7 (RPL7) is a component of the 60S ribosomal subunit, playing a critical role in ribosome assembly and protein synthesis. As part of the large ribosomal subunit, RPL7 contributes to the structural stability of the ribosome and facilitates interactions during translation, such as mRNA decoding and peptide bond formation. Beyond its canonical role in translation, RPL7 has been implicated in extraribosomal functions, including regulation of gene expression, cell cycle control, and apoptosis, often through interactions with specific RNAs or proteins.

Recombinant RPL7 protein is engineered using genetic cloning techniques, where the *RPL7* gene is expressed in heterologous systems like *E. coli* or mammalian cells (e.g., HEK293). This allows for high-yield production of the protein with defined post-translational modifications, depending on the host system. Purification methods typically involve affinity chromatography (e.g., His-tag systems) followed by validation via SDS-PAGE and Western blotting.

Studies utilizing recombinant RPL7 have advanced understanding of ribosome biogenesis defects linked to diseases such as cancer and developmental disorders. For example, dysregulation of RPL7 is observed in certain cancers, where it may act as a tumor suppressor or oncogene depending on cellular context. Additionally, recombinant RPL7 serves as a tool to investigate ribosomopathies and to screen for therapeutic agents targeting ribosomal dysfunction. Its role in viral replication, particularly in RNA viruses that hijack ribosomal machinery, has also been explored.

Overall, recombinant RPL7 provides a versatile platform for dissecting ribosomal biology, disease mechanisms, and potential interventions.

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