| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HBZ |
| Uniprot No | P02008 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-142aa |
| 氨基酸序列 | SLTKTERTIIVSMWAKISTQADTIGTETLERLFLSHPQTKTYFPHFDLHPGSAQLRAHGSKVVAAVGDAVKSIDDIGGALSKLSELHAYILRVDPVNFKLLSHCLLVTLAARFPADFTAEAHAAWDKFLSVVSSVLTEKYR |
| 预测分子量 | 42.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HBZ重组蛋白的3篇代表性文献摘要概括(模拟示例，非真实文献)：
1. **《HTLV-1 HBZ重组蛋白调控宿主细胞转录机制研究》**
作者：Shimazu T, et al.
摘要：研究利用大肠杆菌表达系统制备重组HBZ蛋白，证实其通过抑制宿主细胞CREB信号通路，干扰病毒自身Tax蛋白的转录激活功能，促进HTLV-1病毒潜伏感染。
2. **《重组HBZ蛋白的致癌性功能验证》**
作者：Zhao L, et al.
摘要：通过哺乳动物细胞表达纯化的HBZ重组蛋白，发现其通过结合p300/CBP共激活因子，激活AP-1通路，诱导T细胞异常增殖，揭示了HBZ在成人T细胞白血病中的关键作用。
3. **《HBZ重组蛋白结构解析及其免疫逃逸机制》**
作者：Mori N, et al.
摘要：利用X射线晶体学解析HBZ重组蛋白的C端结构域，发现其通过模拟宿主细胞转录因子形成异源二聚体，抑制MHC-I类分子表达，帮助病毒逃避免疫清除。
注：以上内容为模拟文献概括，实际研究中建议通过PubMed或Web of Science检索真实文献(关键词：HTLV-1 HBZ recombinant protein)。
The HTLV-1 basic leucine zipper (HBZ) protein is a critical regulatory factor encoded by the human T-cell leukemia virus type 1 (HTLV-1), a retrovirus linked to adult T-cell leukemia/lymphoma (ATLL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Unlike most viral proteins, HBZ is transcribed from the antisense strand of the proviral DNA, enabling its persistent expression even during viral latency. This unique feature allows HBZ to manipulate host cell signaling and immune evasion mechanisms, contributing to HTLV-1 pathogenesis.
HBZ contains an N-terminal activation domain and a C-terminal basic leucine zipper (bZIP) domain, which facilitates interactions with host transcription factors, including members of the CREB/ATF family. These interactions disrupt normal transcriptional regulation, promoting cell proliferation and inhibiting apoptosis. HBZ also modulates pathways such as TGF-β signaling and the NF-κB cascade, further enhancing viral persistence and leukemogenesis. Notably, HBZ suppresses viral replication by antagonizing the function of the viral transactivator Tax, creating a balance that favors chronic infection over immune detection.
Recombinant HBZ protein, typically produced in bacterial or mammalian expression systems, is a vital tool for studying its molecular interactions and pathogenic mechanisms. Researchers use purified HBZ to investigate its structure-function relationships, host protein binding partners, and immunomodulatory effects. Additionally, recombinant HBZ aids in developing diagnostic assays and therapeutic strategies, such as targeted inhibitors or vaccines. Its role in immune evasion and oncogenesis makes HBZ a focus for understanding HTLV-1-related diseases and exploring novel antiviral interventions. Despite progress, the complexity of HBZ’s dual roles in viral persistence and cellular transformation remains an active area of study.
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