| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Ptger4 |
| Uniprot No | P35408 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-488aa |
| 氨基酸序列 | MSTPGVNSSASLSPDRLNSPVTIPAVMFIFGVVGNLVAIVVLCKSRKEQKETTFYTLVCGLAVTDLLGTLLVSPVTIATYMKGQWPGGQPLCEYSTFILLFFSLSGLSIICAMSVERYLAINHAYFYSHYVDKRLAGLTLFAVYASNVLFCALPNMGLGSSRLQYPDTWCFIDWTTNVTAHAAYSYMYAGFSSFLILATVLCNVLVCGALLRMHRQFMRRTSLGTEQHHAAAAASVASRGHPAASPALPRLSDFRRRRSFRRIAGAEIQMVILLIATSLVVLICSIPLVVRVFVNQLYQPSLEREVSKNPDLQAIRIASVNPILDPWIYILLRKTVLSKAIEKIKCLFCRIGGSRRERSGQHCSDSQRTSSAMSGHSRSFISRELKEISSTSQTLLPDLSLPDLSENGLGGRNLLPGVPGMGLAQEDTTSLRTLRISETSDSSQGQDSESVLLVDEAGGSGRAGPAPKGSSLQVTFPSETLNLSEKCI |
| 预测分子量 | 53,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Ptger4(EP4受体)重组蛋白研究的3篇代表性文献摘要示例(注：文献为模拟示例，非真实存在)：
---
1. **文献名称**：Expression and functional characterization of recombinant human prostaglandin E2 receptor EP4 in HEK293 cells
**作者**：Nakamura K, et al.
**摘要**：该研究成功在HEK293细胞中表达了重组人源EP4受体，通过体外信号通路分析揭示了EP4通过cAMP/PKA途径调控炎症相关基因的表达，为靶向EP4的抗炎药物开发提供模型。
2. **文献名称**：Crystal structure of the EP4 receptor bound to an antagonist
**作者**：Sakuma Y, et al.
**摘要**：利用昆虫细胞表达系统获得高纯度EP4重组蛋白，解析其与选择性拮抗剂的复合物晶体结构，揭示了配体结合域的关键氨基酸残基，指导新型EP4抑制剂的理性设计。
3. **文献名称**：Recombinant EP4 receptor-mediated modulation of colorectal cancer cell proliferation
**作者**：Li J, et al.
**摘要**：研究通过大肠杆菌表达系统制备EP4胞外域重组蛋白，证实其可竞争性抑制PGE2与肿瘤细胞表面EP4结合，显著降低结肠癌细胞增殖和迁移能力。
---
**注**：以上文献为示例，实际文献需通过PubMed/Google Scholar以关键词“Ptger4 recombinant”、“EP4 receptor expression”检索获取，并核实摘要内容。
**Background of PTGER4 Recombinant Protein**
Prostaglandin E receptor 4 (PTGER4), also known as EP4. is a G protein-coupled receptor (GPCR) that binds prostaglandin E2 (PGE2), a lipid mediator involved in inflammation, immune regulation, and pain signaling. As one of four PGE2 receptor subtypes (EP1-EP4), EP4 is distinguished by its role in modulating cyclic AMP (cAMP) signaling and activating downstream pathways such as MAPK/ERK, contributing to cellular processes like proliferation, differentiation, and apoptosis.
Recombinant PTGER4 protein is engineered for research and therapeutic applications. Produced using expression systems like mammalian cells (e.g., HEK293) or insect cells (e.g., Sf9), the recombinant protein retains native conformational and functional properties. Purification techniques, including affinity chromatography with tags like His or FLAG, ensure high purity and bioactivity.
EP4 is implicated in chronic inflammatory diseases (e.g., rheumatoid arthritis), cancer progression (e.g., tumor angiogenesis and immune evasion), and cardiovascular conditions. Its recombinant form enables *in vitro* studies to dissect signaling mechanisms, screen antagonists/agonists, and develop targeted therapies. For example, EP4 antagonists are explored for anti-inflammatory and anticancer effects, though challenges like systemic side effects persist.
Structural studies using recombinant PTGER4. often combined with cryo-EM or X-ray crystallography, have advanced understanding of ligand-binding dynamics and receptor activation, aiding rational drug design. Additionally, EP4's role in tissue repair and homeostasis highlights its therapeutic potential in regenerative medicine.
In summary, PTGER4 recombinant protein serves as a critical tool for unraveling PGE2-mediated pathways and developing precision therapies, bridging molecular insights with clinical applications.
×
