| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPACA3 |
| Uniprot No | Q8IXA5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-215aa |
| 氨基酸序列 | MVSALRGAPLIRVHSSPVSSPSVSGPRRLVSCLSSQSSALSQSGGGSTSAAGIEARSRALRRRWCPAGIMLLALVCLLSCLLPSSEAKLYGRCELARVLHDFGLDGYRGYSLADWVCLAYFTSGFNAAALDYEADGSTNNGIFQINSRRWCSNLTPNVPNVCRMYCSDLLNPNLKDTVICAMKITQEPQGLGYWEAWRHHCQGKDLTEWVDGCDF |
| 预测分子量 | 23,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPACA3重组蛋白的模拟参考文献示例(仅供学术参考，具体文献需通过数据库查询)：
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1. **文献名称**：*Recombinant SPACA3 Expression in E. coli and Its Role in Sperm-Zona Pellucida Binding*
**作者**：Yamaguchi R. et al.
**摘要**：本研究成功构建了SPACA3重组蛋白的原核表达系统，纯化后验证其与透明带蛋白(ZP)的特异性结合能力，提示其在受精过程中的潜在作用。
2. **文献名称**：*Structural Characterization of SPACA3 and Its Implications in Male Fertility*
**作者**：Li X. & Wang H.
**摘要**：通过重组SPACA3蛋白的晶体结构解析，揭示了其与顶体酶活性的关联，为男性不育症的分子机制提供了新见解。
3. **文献名称**：*SPACA3 Recombinant Protein as a Potential Biomarker for Asthenozoospermia*
**作者**：Chen L. et al.
**摘要**：利用重组SPACA3蛋白制备抗体，发现弱精症患者精液中SPACA3表达显著降低，提示其作为临床诊断标志物的潜力。
4. **文献名称**：*Functional Analysis of SPACA3 in Sperm Acrosome Reaction Using Recombinant Protein Models*
**作者**：Kato S. et al.
**摘要**：通过体外重组SPACA3功能实验，证实其参与钙离子介导的顶体反应调控，影响精子穿透卵母细胞的能力。
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**注意**：以上文献为示例性内容，实际研究需通过PubMed、Web of Science等平台检索关键词“SPACA3 recombinant protein”获取。
**Background of SPACA3 Recombinant Protein**
SPACA3 (Sperm Acrosome Associated Protein 3) is a testis-specific protein predominantly expressed in mammalian sperm cells. It localizes to the acrosome, a specialized organelle critical for sperm-egg interaction during fertilization. SPACA3 is implicated in acrosomal biogenesis, maturation, or the acrosome reaction—a process enabling sperm to penetrate the zona pellucida of the oocyte. Structurally, it belongs to the SPACA protein family, characterized by conserved cysteine-rich domains that may mediate protein-protein interactions or enzymatic functions.
The recombinant SPACA3 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems, enabling large-scale production for functional studies. Its recombinant form retains biological activity, allowing researchers to investigate its role in sperm physiology, including its potential involvement in membrane fusion, ion channel regulation, or interaction with egg surface receptors. Dysregulation of SPACA3 has been linked to male infertility in animal models, suggesting its utility as a biomarker for diagnosing sperm dysfunction.
Studies using SPACA3 recombinant protein also explore its application in contraceptive development, as targeting acrosomal proteins could disrupt fertilization. Additionally, it serves as an antigen for antibody generation, aiding in diagnostic assays or imaging sperm morphology. Recent advances in proteomics and CRISPR-based gene editing have further highlighted SPACA3's regulatory mechanisms, positioning it as a promising target for both reproductive research and clinical interventions in infertility.
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