| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDDC3 |
| Uniprot No | Q8N4P3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-140aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHLDEVELHFGAQVRRLVEEVTDDKTLPKLER KRLQVEQAPHSSPGAKLVKLADKLYNLRDLNRCTPEVKIQMGSEAAQLLE AADFAARKHRQQRRKDPEGTPYINHPIGVARILTHEAGITDIVVLQAALL HDTVEDTDTT |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDDC3重组蛋白的模拟参考文献示例(注：HDDC3相关研究较为有限，以下内容为假设性示例，建议通过学术数据库核实真实文献)：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant HDDC3 Protein in *E. coli*"
**作者**: Zhang L, Wang Y, Chen X
**摘要**: 本研究报道了在大肠杆菌系统中高效表达并纯化HDDC3重组蛋白的优化方法。通过亲和层析获得高纯度蛋白，并证实其具有ATP水解酶活性，为后续功能研究奠定基础。
2. **文献名称**: "Structural Insights into HDDC3's Role in Chromatin Remodeling"
**作者**: Smith J, Tanaka K, Lee S
**摘要**: 利用X射线晶体学解析了HDDC3蛋白的晶体结构，发现其C端结构域与组蛋白H3特异性结合，提示其可能通过表观遗传调控参与基因沉默通路。
3. **文献名称**: "HDDC3 Knockdown Impairs DNA Repair via Recombinant Protein Interaction Screening"
**作者**: Gupta R, Müller P, Wei H
**摘要**: 通过酵母双杂交筛选发现HDDC3与BRCA1复合物存在相互作用，重组HDDC3蛋白的体外实验表明其可增强DNA损伤修复效率，提示其在基因组稳定性中的潜在作用。
4. **文献名称**: "HDDC3 Recombinant Protein Attenuates Inflammatory Response in Macrophages"
**作者**: Kim M, Park J, Rossi F
**摘要**: 在哺乳动物细胞中表达HDDC3重组蛋白，发现其通过抑制NF-κB信号通路减少炎症因子释放，为治疗自身免疫疾病提供了新靶点。
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建议通过 **PubMed** 或 **Web of Science** 检索最新文献，关键词可尝试 "HDDC3 recombinant" 或结合其功能(如染色质修饰、酶活性等)。若需具体领域文献，请补充说明研究方向。
**Background of HDDC3 Recombinant Protein**
HDDC3 (Histone Deacetylase Domain-Containing Protein 3) is a member of the histone deacetylase (HDAC)-related protein family, which plays a critical role in epigenetic regulation by modifying chromatin structure and gene expression. Unlike classical HDACs, HDDC3 lacks full catalytic activity but retains structural motifs associated with histone deacetylase function, suggesting its potential involvement in chromatin remodeling or interaction with other regulatory complexes. The protein is conserved across eukaryotes, highlighting its biological significance, though its precise molecular mechanisms remain less characterized compared to other HDAC family members.
Recombinant HDDC3 protein is engineered through genetic cloning and expression systems, such as *E. coli* or mammalian cell cultures, to produce a purified, functional form for experimental studies. This recombinant tool enables researchers to investigate HDDC3's interactions with DNA, histones, or partner proteins, as well as its role in cellular processes like differentiation, apoptosis, or stress responses. Studies suggest HDDC3 may influence transcriptional repression, genome stability, or metabolic pathways, with emerging links to diseases such as cancer or neurodegenerative disorders.
The development of HDDC3 recombinant protein has advanced structural studies (e.g., crystallography) and biochemical assays (e.g., binding affinity tests), providing insights into its unique regulatory functions. Further research aims to clarify its enzymatic potential, therapeutic relevance, and utility as a biomarker. By leveraging recombinant HDDC3. scientists aim to unravel its contributions to epigenetic networks and disease pathology, bridging gaps in understanding non-catalytic HDAC-related proteins.
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