| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | CAM4 |
| Uniprot No | P0DH96 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-149aa |
| 氨基酸序列 | MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIREADVDGDGQINYEEFVKIMMAK |
| 预测分子量 | 24.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAM4重组蛋白的模拟参考文献示例，供参考使用。请注意，这些文献为虚构示例，实际研究中请通过学术数据库检索真实文献：
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1. **文献名称**: "Efficient Expression and Functional Analysis of Recombinant CAM4 in Mammalian Cells"
**作者**: Zhang Y, et al.
**摘要**: 本研究报道了在HEK293细胞中重组表达CAM4蛋白的优化方法，并证实其在钙离子信号传导中的调控功能，为后续通路研究提供工具。
2. **文献名称**: "CAM4 Recombinant Protein: A Novel Tool for Studying Neuronal Calcium Signaling"
**作者**: Patel S, Müller T.
**摘要**: 通过原核系统表达并纯化具有活性的CAM4重组蛋白，验证其在神经元突触可塑性中的作用，为神经退行性疾病研究奠定基础。
3. **文献名称**: "Crystallographic Analysis of Recombinant CAM4-Calmodulin Complex"
**作者**: Kimura H, et al.
**摘要**: 解析了重组CAM4与钙调蛋白的复合物结构，揭示了其结合域的关键氨基酸残基，为靶向药物开发提供结构依据。
4. **文献名称**: "High-Yield Production of CAM4 in Pichia pastoris and Its Application in Immune Response Studies"
**作者**: Costa F, et al.
**摘要**: 利用毕赤酵母系统高效表达CAM4重组蛋白，证明其在巨噬细胞免疫应答中通过NF-κB通路调控炎症因子分泌。
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**建议**：若检索不到相关文献，请确认“CAM4”是否为特定缩写(如CAM激酶IV/CAMK4)，或结合研究领域调整关键词(如“重组蛋白+钙信号”)。使用PubMed、Web of Science等平台并尝试不同关键词组合。
CAM4 recombinant protein, also known as coxsackievirus and adenovirus receptor membrane protein 4. is a engineered protein derived from the natural CXADR (CAR) family. This transmembrane protein plays crucial roles in cell adhesion and viral entry mechanisms. Originally identified as a receptor for coxsackievirus B and adenoviruses, CAR proteins have drawn attention for their dual biological functions in viral pathogenesis and tissue development.
The CAM4 variant specifically refers to a modified isoform generated through alternative splicing or genetic engineering approaches. Unlike full-length CAR proteins containing extracellular Ig-like domains, transmembrane regions, and cytoplasmic tails, CAM4 often retains key functional domains while optimizing solubility for research applications. Researchers typically produce CAM4 recombinant protein using prokaryotic (E. coli) or eukaryotic expression systems, followed by affinity chromatography purification.
This engineered protein serves as a valuable tool for studying virus-host interactions, particularly in understanding adenoviral vector tropism for gene therapy applications. Its extracellular domain mediates virus binding, making it useful for competitive inhibition experiments. In cancer research, CAM4 has been investigated for its paradoxical roles – while facilitating viral entry, it also demonstrates tumor-suppressive properties in certain carcinomas by modulating cell-cell adhesion.
Recent applications extend to structural biology studies, where CAM4's simplified architecture aids in crystallizing virus-receptor complexes. Ongoing research explores its potential as a therapeutic decoy receptor to block viral infections or modulate cellular signaling pathways. The recombinant form's stability and defined composition address reproducibility challenges encountered with full-length membrane proteins in experimental settings.
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