| 纯度 | >90%SDS-PAGE. |
| 种属 | Erwinia |
| 靶点 | pagC |
| Uniprot No | D2T690 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-185aa |
| 氨基酸序列 | DSHALSIGYAQSRVQDFKNLRGVNLKYRYEPNSLLGVITSFSYMSAGGHEFDSLSWGDTYYDDRIKVKYYSLLIGPSYRINKYVSLYAVSGLGSSKLDLTQNYRHTNYTYTEHTASNTTSFAYGAGVQINPLKNIAIDISYEKSKINAKKINGFSMGIGYHF |
| 预测分子量 | 25.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于pagC重组蛋白的参考文献概览：
1. **文献名称**: "Molecular characterization and functional analysis of the Salmonella PagC protein"
**作者**: Miller, S.I., et al.
**摘要**: 该研究首次克隆并表达了沙门氏菌pagC基因，发现PagC是一种外膜蛋白，通过基因敲除实验证明其对细菌在巨噬细胞内生存至关重要，揭示了PagC在宿主细胞内抵抗抗菌肽的作用机制。
2. **文献名称**: "Recombinant PagC protein induces protective immunity against Salmonella infection"
**作者**: Zhang, Y., et al.
**摘要**: 通过大肠杆菌表达系统成功制备重组PagC蛋白，动物实验表明该蛋白能显著提高小鼠对沙门氏菌感染的抗体水平，证实其作为亚单位疫苗候选抗原的潜力。
3. **文献名称**: "Structural insights into PagC-mediated membrane interaction in bacterial pathogenesis"
**作者**: Gupta, R., & Mondragón, A.
**摘要**: 利用X射线晶体学解析了重组PagC蛋白的三维结构，发现其具有典型的β-桶状外膜蛋白特征，通过体外脂质体结合实验阐明了PagC与宿主细胞膜相互作用的分子基础。
注：以上为示例性内容，实际文献需通过PubMed或Web of Science等数据库检索。建议使用关键词"pagC recombinant protein"+"Salmonella"获取最新研究。
PagC recombinant protein is a genetically engineered protein derived from the PagC gene, originally identified in pathogenic bacteria such as *Salmonella enterica*. PagC is a virulence-associated outer membrane protein that plays a critical role in bacterial survival and host-pathogen interactions. It is implicated in facilitating bacterial invasion of host cells, immune evasion, and resistance to host defense mechanisms. The protein is regulated by the PhoP/PhoQ two-component system, which is activated under host microenvironmental conditions, such as low magnesium or acidic pH, mimicking the intracellular environment of macrophages.
Recombinant PagC is produced by cloning the pagC gene into expression vectors (e.g., *E. coli*), followed by purification using affinity chromatography. This engineered protein retains the functional domains of native PagC, including its β-barrel structure and surface-exposed loops, which are essential for its interaction with host receptors or immune components. Researchers utilize recombinant PagC to study bacterial pathogenesis, host immune responses, and to develop therapeutic or diagnostic tools. Its immunogenic properties make it a candidate for subunit vaccines against *Salmonella* and other Gram-negative pathogens.
Studies have also explored PagC's role in biofilm formation, antimicrobial resistance, and its potential as a biomarker for infection. Structural analysis of recombinant PagC aids in mapping epitopes for antibody development or designing inhibitors to block its virulence functions. Despite its pathogenic origins, PagC serves as a valuable model for understanding bacterial outer membrane protein dynamics and host adaptation strategies, contributing to broader applications in microbiology and immunology research.
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