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Recombinant Human xylF protein

  • 中文名: D-木糖结合周质蛋白(xylF)重组蛋白
  • 别    名: xylF;xylT;D-xylose-binding periplasmic protein
货号: PA2000-4782
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点xylF
Uniprot No P37387
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间 24-330aa
氨基酸序列KEVKIGM AIDDLRLERW QKDRDIFVKK AESLGAKVFV QSANGNEETQ MSQIENMINR GVDVLVIIPY NGQVLSNVVK EAKQEGIKVL AYDRMINDAD IDFYISFDNE KVGELQAKAL VDIVPQGNYF LMGGSPVDNN AKLFRAGQMK VLKPYVDSGK IKVVGDQWVD GWLPENALKI MENALTANNN KIDAVVASND ATAGGAIQAL SAQGLSGKVA ISGQDADLAG IKRIAAGTQT MTVYKPITLL ANTAAEIAVE LGNGQEPKAD TTLNNGLKDV PSRLLTPIDV NKNNIKDTVI KDGFHKESEL
预测分子量35,7 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于xylF重组蛋白的3-4条参考文献示例(内容基于公开研究整合,非真实文献):

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1. **《Functional Characterization of XylF as a Xylose-Binding Protein in Escherichia coli》**

*作者:Rogers, P. et al.*

摘要:研究报道了xylF基因在大肠杆菌中的重组表达及纯化,证实XylF蛋白作为木糖转运系统的关键组分,通过结合实验验证其特异性识别木糖,为木糖代谢机制提供分子基础。

2. **《Cloning and Expression of Xylose Isomerase Pathway Genes (xylA and xylF) in Saccharomyces cerevisiae》**

*作者:Zhang, Y. et al.*

摘要:通过重组技术将xylF与xylA基因共表达于酵母中,显著提高木糖异构化效率,为利用木质纤维素生产乙醇的工程菌株优化提供新策略。

3. **《Structural Analysis of Recombinant XylF Protein from Thermus thermophilus》**

*作者:Lorenz, M. et al.*

摘要:解析了嗜热菌来源的重组XylF蛋白的晶体结构,揭示其木糖结合位点的构象变化,为设计耐高温木糖代谢酶提供结构生物学依据。

4. **《Enhancing Xylose Utilization in Bacillus subtilis via Heterologous Expression of XylF》**

*作者:Ahmed, S. et al.*

摘要:将重组XylF蛋白导入枯草芽孢杆菌,显著提升菌株对木糖的摄取能力,推动其在生物燃料工业中的应用潜力。

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以上文献示例聚焦于xylF重组蛋白的功能解析、结构研究及工业应用,具体内容需根据实际研究补充。如需真实文献,建议通过PubMed或Web of Science检索关键词“xylF recombinant protein”。

背景信息

**Background of XylF Recombinant Protein**

The XylF protein is a key component of the xylose uptake system in various bacteria, particularly in *Escherichia coli* and related species. It functions as a substrate-binding subunit of the XylFGHR ATP-binding cassette (ABC) transporter, which is responsible for high-affinity uptake of D-xylose, a pentose sugar commonly found in plant biomass. Xylose metabolism is critical for microbial growth on lignocellulosic materials, making XylF relevant in biofuel production and biotechnological applications where xylose serves as a carbon source.

Recombinant XylF is produced by cloning the *xylF* gene into expression vectors (e.g., plasmid systems under inducible promoters like T7 or arabinose) and expressing it in heterologous hosts such as *E. coli*. This allows large-scale purification for functional or structural studies. The protein’s ability to bind xylose with high specificity has driven interest in engineering or optimizing its properties to enhance xylose utilization in industrial microbes, particularly for bioethanol production from agricultural waste.

Structural analyses of XylF, often via X-ray crystallography or cryo-EM, reveal conserved features of sugar-binding proteins, including a characteristic bilobed domain that closes upon ligand binding. These studies provide insights into substrate recognition mechanisms and guide mutagenesis efforts to alter substrate affinity or thermal stability. Additionally, recombinant XylF serves as a model for studying ABC transporter dynamics and microbial adaptation to environmental carbon sources.

Efforts to improve recombinant __expression (e.g., codon optimization, fusion tags) and purification protocols aim to support its application in synthetic biology and enzyme engineering, aligning with broader goals of sustainable bioproduction and renewable energy.

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