| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GLUR2; GLURB; GluA2; HBGR2; GluR-K2 |
| Entrez GeneID | 2891 |
| clone | 7A7A3 |
| WB Predicted band size | 99kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GRIA2 (AA: 35-175) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于GRIA2抗体的3篇参考文献示例(内容为模拟，仅供参考)：
1. **文献名称**：*"Selective loss of GluA2 AMPA receptors in Alzheimer’s disease"*
**作者**：Smith A, et al.
**摘要**：研究利用GRIA2特异性抗体进行Western blot和免疫组化，发现阿尔茨海默病患者海马区GluA2蛋白水平显著降低，提示其与突触功能障碍的关联。
2. **文献名称**：*"GRIA2 antibody validation for immunohistochemical mapping in rodent brain"*
**作者**：Chen L, et al.
**摘要**：该研究系统验证了多种GRIA2抗体的特异性，通过敲除小鼠模型证实抗体信号的特异性，并绘制了GluA2在大鼠皮层及海马的分布图谱。
3. **文献名称**：*"AMPA receptor subunit trafficking in ischemia: Role of GRIA2 antibodies in live-cell imaging"*
**作者**：Kimura T, et al.
**摘要**：采用GRIA2抗体标记活神经元，发现脑缺血模型中GluA2亚基的内吞增加，揭示了其与神经元兴奋性毒性相关的分子机制。
4. **文献名称**：*"Autoantibodies against GRIA2 in autoimmune encephalitis: clinical correlations"*
**作者**：Zhang Y, et al.
**摘要**：通过ELISA和细胞免疫荧光法，发现部分自身免疫性脑炎患者血清中存在GRIA2抗体，提示其可能干扰AMPA受体功能并导致神经症状。
(注：以上文献为示例性质，实际引用时需核实真实文献信息。)
The GRIA2 antibody targets the glutamate ionotropic receptor AMPA type subunit 2 (GluA2), encoded by the GRIA2 gene. GluA2 is a critical component of AMPA receptors, which are ligand-gated ion channels mediating fast excitatory synaptic transmission in the central nervous system. These receptors play essential roles in synaptic plasticity, learning, and memory. GRIA2 antibodies are widely used in neuroscience research to study receptor expression, localization, and function in both physiological and pathological contexts. They are applied in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate GluA2 distribution in brain tissues or cultured neurons.
GluA2 is notable for its role in regulating calcium permeability. Most AMPA receptors containing GluA2 are impermeable to calcium due to RNA editing at the Q/R site in its transmembrane domain. Dysregulation of GluA2 expression or editing has been implicated in neurological disorders, including Alzheimer’s disease, epilepsy, and ischemic brain injury. Antibodies specific to GRIA2 help researchers examine changes in receptor composition during disease progression or in response to pharmacological interventions. Some antibodies distinguish between edited (R) and unedited (Q) isoforms, enabling studies on RNA editing mechanisms. Validation of GRIA2 antibodies often involves knockout controls or peptide blocking to confirm specificity, given the high homology among AMPA receptor subunits. These tools continue to advance understanding of excitatory signaling pathways and their therapeutic targeting.
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