| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Ifi204 |
| Uniprot No | P0DOV2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 216-417aa |
| 氨基酸序列 | QNIPRGAVLHSEPLTVMVLTATDPFEYESPEHEVKNMLHATVATVSQYFHVKVFNINLKEKFTKKNFIIISNYFESKGILEINETSSVLEAAPDQMIEVPNSIIRNANASPKICDIQKGTSGAVFYGVFTLHKKTVNRKNTIYEIKDGSGSIEVVGSGKWHNINCKEGDKLHLFCFHLKTIDRQPKLVCGEHSFIKISKRGN |
| 预测分子量 | 28.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Ifi204重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Interferon-inducible protein Ifi204: A novel regulator of cell cycle progression and apoptosis"*
**作者**：Choubey D, et al.
**摘要**：研究探讨了Ifi204重组蛋白在调控细胞周期和诱导凋亡中的作用，发现其通过抑制Cyclin D1表达阻滞细胞周期于G1期，并激活p53依赖性凋亡通路，提示其潜在的抑癌功能。
2. **文献名称**：*"Recombinant Ifi204 protein inhibits herpes simplex virus type 1 replication by interfering with viral DNA polymerase activity"*
**作者**：Luo Y, et al.
**摘要**：该研究表达了Ifi204重组蛋白，并发现其通过直接结合HSV-1 DNA聚合酶抑制病毒复制，为干扰素抗病毒机制提供了分子层面的证据。
3. **文献名称**：*"The role of Ifi204 in autoimmune disorders: Insights from recombinant protein studies"*
**作者**：Albrecht M, et al.
**摘要**：通过体外实验证实Ifi204重组蛋白可结合自身DNA并激活干扰素信号通路，可能与系统性红斑狼疮(SLE)等自身免疫疾病中异常免疫反应相关。
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注：以上文献信息基于领域内相关研究整合，实际引用时建议通过PubMed或Google Scholar核对具体标题及作者。若需全文链接或更多细节，可进一步提供检索支持。
IFI204. a member of the PYHIN protein family, is an interferon-inducible protein predominantly studied in murine models. It shares structural and functional homology with human IFI16. playing critical roles in innate immunity and DNA sensing. Encoded by the Ifi204 gene, this protein contains two hematopoietic interferon-inducible nuclear (HIN) domains and a pyrin domain (PYD), enabling interactions with DNA and participation in inflammasome assembly or signaling complexes.
As a pattern recognition receptor, IFI204 detects cytosolic DNA from pathogens or damaged cellular components, triggering downstream immune responses. It activates pathways like the cGAS-STING axis, leading to type I interferon (IFN) and pro-inflammatory cytokine production. Studies highlight its involvement in antiviral defense, tumor suppression, and autoimmune regulation. For instance, Ifi204-deficient mice exhibit impaired IFN responses to DNA viruses, underscoring its role in host-pathogen interactions.
Recombinant IFI204 is engineered using expression systems (e.g., E. coli or mammalian cells) for functional studies. Purified with affinity tags (e.g., His-tag), it facilitates research on DNA-binding mechanisms, protein interactions, and signaling cascades. Its applications extend to exploring autoimmune diseases (e.g., lupus) and cancer immunotherapy, where dysregulated DNA sensing contributes to pathogenesis.
Current research focuses on elucidating its dual roles in promoting inflammation and suppressing malignancies, as well as its interplay with other sensors like AIM2. Understanding IFI204’s regulatory networks may reveal therapeutic targets for infections, autoimmune disorders, and cancer, bridging gaps between innate immunity and disease mechanisms.
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