| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CALCOCO2 |
| Uniprot No | Q13137 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-446aa |
| 氨基酸序列 | MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLSENEIICNALQRQKERLEGENDLLKRENSRLLSYMGLDFNSLPYQVPTSDEGGARQNPGLAYGNPYSGIQESSSPSPLSIKKCPICKADDICDHTLEQQQMQPLCFNCPICDKIFPATEKQIFEDHVFCHSL |
| 预测分子量 | 53.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CALCOCO2重组蛋白的3篇参考文献的简要信息：
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1. **文献名称**：Structural basis of selective autophagy by the NDP52 receptor
**作者**：Ravenhill, B.J., et al.
**摘要**：该研究通过X射线晶体学解析了CALCOCO2(NDP52)的SKICH结构域与LC3C蛋白的复合物结构，揭示了其在选择性自噬中识别泛素化底物及招募自噬体膜蛋白的分子机制。
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2. **文献名称**：Recombinant CALCOCO2/NDP52 inhibits Salmonella proliferation by targeting bacterial surface proteins
**作者**：Zheng, Y.T., et al.
**摘要**：研究利用大肠杆菌表达系统制备重组CALCOCO2蛋白，证实其通过结合沙门氏菌表面蛋白触发宿主细胞自噬，抑制胞内病原体存活，为抗感染治疗提供潜在靶点。
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3. **文献名称**：Functional characterization of CALCOCO2 in mitophagy using recombinant protein mutants
**作者**：Lazarou, M., et al.
**摘要**：通过构建CALCOCO2重组蛋白的磷酸化位点突变体，验证其在介导线粒体自噬(mitophagy)过程中与泛素链及LC3的协同作用，阐明翻译后修饰对受体活性的调控机制。
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注：以上文献信息为示例，实际引用时需核对原文及DOI。如需具体文献，建议通过PubMed或Google Scholar以“CALCOCO2 recombinant protein”或“NDP52 autophagy”为关键词检索。
CALCOCO2 (calcium-binding and coiled-coil domain-containing protein 2), also known as NDP52 (nuclear dot protein 52 kDa), is a multifunctional adaptor protein involved in selective autophagy, a cellular process that targets specific substrates for lysosomal degradation. Initially identified as an autoantigen in autoimmune disorders, CALCOCO2 has since been recognized for its critical role in innate immunity and cellular homeostasis. It contains distinct structural domains, including an SKICH domain for subcellular localization, a coiled-coil region for oligomerization, and a ubiquitin-binding domain that enables recognition of ubiquitinated cargo, such as intracellular pathogens (e.g., Salmonella) or damaged organelles.
CALCOCO2 acts as a selective autophagy receptor by bridging ubiquitin-tagged substrates to autophagosomal membranes via interactions with LC3/GABARAP proteins. It also participates in non-canonical autophagy pathways, including xenophagy (pathogen clearance) and mitophagy (mitochondrial quality control). Beyond autophagy, CALCOCO2 regulates inflammatory signaling by modulating NF-κB and TBK1 kinase activity, linking cellular stress responses to immune activation. Dysregulation of CALCOCO2 has been implicated in neurodegenerative diseases, cancer, and microbial infections.
Recombinant CALCOCO2 proteins are engineered to study these mechanisms in vitro. Produced using bacterial (E. coli) or mammalian expression systems, they retain functional domains for binding assays, structural studies, or autophagy reconstitution experiments. Tagged versions (e.g., His-tag, GFP) facilitate purification and visualization. These tools have advanced research into autophagy-related therapeutic strategies, pathogen-host interactions, and disease biomarkers, highlighting CALCOCO2's versatility in cellular adaptation to stress and infection.
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