| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HHEX |
| Uniprot No | Q03014 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-270aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMQYPHPG PAAGAVGVPL YAPTPLLQPA HPTPFYIEDI LGRGPAAPTP APTLPSPNSS FTSLVSPYRT PVYEPTPIHP AFSHHSAAAL AAAYGPGGFG GPLYPFPRTV NDYTHALLRH DPLGKPLLWS PFLQRPLHKR KGGQVRFSND QTIELEKKFE TQKYLSPPER KRLAKMLQLS ERQVKTWFQN RRAKWRRLKQ ENPQSNKKEE LESLDSSCDQ RQDLPSEQNK GASLDSSQCS PSPASQEDLE SEISEDSDQE VDIEGDKSYF NAG |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HHEX重组蛋白研究的示例参考文献(注：以下为虚拟文献示例，实际引用请核实真实来源)：
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1. **文献名称**: *HHEX Recombinant Protein Suppresses Leukemia Cell Proliferation via Transcriptional Repression*
**作者**: Tanaka, K. et al. (2020)
**摘要**: 本研究利用大肠杆菌表达系统纯化HHEX重组蛋白，发现其通过抑制Wnt/β-catenin信号通路关键基因的转录，显著抑制急性髓系白血病细胞的增殖，揭示了HHEX作为肿瘤抑制因子的潜在机制。
2. **文献名称**: *Structural and Functional Analysis of the HHEX DNA-Binding Domain*
**作者**: Müller, R. & Schmidt, E. (2018)
**摘要**: 通过重组表达HHEX蛋白的DNA结合结构域，结合X射线晶体学分析，揭示了其与靶基因启动子区域的特异性结合模式，为理解HHEX在胚胎发育和甲状腺分化中的调控作用提供结构基础。
3. **文献名称**: *HHEX Recombinant Protein Modulates VEGF Signaling in Endothelial Cells*
**作者**: Chen, L. et al. (2021)
**摘要**: 研究证明重组HHEX蛋白可通过竞争性结合VEGF受体胞内结构域，抑制血管内皮细胞的迁移和管腔形成，提示HHEX在血管生成相关疾病治疗中的应用潜力。
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**说明**：实际研究中请通过PubMed、Web of Science等平台检索真实文献(关键词：HHEX recombinant protein, HHEX functional study)。真实文献可能聚焦于HHEX在发育、癌症或代谢调控中的分子机制及其重组蛋白的应用。
**Background of HHEX Recombinant Protein**
The Hematopoietically Expressed Homeobox (HHEX) protein, encoded by the *HHEX* gene, is a member of the homeobox family of transcription factors, which play critical roles in regulating gene expression during embryonic development and tissue differentiation. HHEX is notably involved in the development of hematopoietic, thyroid, liver, and pancreatic tissues. It acts as a transcriptional repressor or activator, depending on cellular context, and participates in key signaling pathways such as Wnt/β-catenin and FGF (fibroblast growth factor), which are essential for organogenesis and cell fate determination.
HHEX is particularly vital in early hematopoiesis, where it regulates the formation of blood cells, and in endocrine organ development, including thyroid morphogenesis. Dysregulation of HHEX has been linked to several pathologies, including leukemia, thyroid dysgenesis, and diabetes, highlighting its importance in both normal physiology and disease.
Recombinant HHEX protein is engineered through molecular cloning and expression in heterologous systems (e.g., *E. coli* or mammalian cells), enabling high-purity production for research applications. This recombinant form retains the functional domains of native HHEX, including the DNA-binding homeodomain, allowing it to interact with target gene promoters. It is widely used to study HHEX’s role in transcriptional regulation, cellular differentiation, and disease mechanisms. Additionally, it serves as a tool for screening therapeutic compounds or deciphering molecular interactions in developmental biology and oncology.
The availability of recombinant HHEX protein has advanced mechanistic studies, offering insights into its dual roles as a developmental regulator and a potential therapeutic target.
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