| WB | 1/250 - 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DNM2;DYN2; CMT2M; DYNII; LCCS5; CMTDI1; CMTDIB; DI-CMTB |
| Entrez GeneID | 1785 |
| clone | 3F5F3 |
| WB Predicted band size | 98kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Dynamin-2 (AA: 520-744) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于Dynamin-2抗体的代表性文献(*模拟示例，实际文献需根据具体研究调整*)：
1. **标题**：*Dynamin-2 mutations associated with centronuclear myopathy cause hyperactive GTPase activity and synaptic vesicle recycling defects*
**作者**：Kenniston JA, et al.
**摘要**：该研究利用特异性Dynamin-2抗体，揭示了与中心核肌病相关的突变导致Dynamin-2 GTP酶活性异常升高，破坏神经肌肉接头囊泡循环，为疾病机制提供分子基础。
2. **标题**：*Dynamin-2 regulates actin dynamics and microtubule organization in podocytes*
**作者**：Shibata T, et al.
**摘要**：通过免疫荧光和抗体阻断实验，证明Dynamin-2通过调控细胞骨架蛋白(如cofilin)的活性，影响足细胞形态和肾脏滤过功能，提示其在肾病中的潜在作用。
3. **标题**：*Antibody-based targeting of dynamin-2 enhances endocytic uptake in cancer cells*
**作者**：Chen Z, et al.
**摘要**：开发了一种靶向Dynamin-2胞内结构域的单克隆抗体，发现其可特异性抑制肿瘤细胞内吞作用，降低EGFR信号传导，为癌症靶向治疗提供新策略。
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**注**：以上文献为模拟概括，实际引用请通过PubMed/Google Scholar以"Dynamin-2 antibody"+"具体研究领域"检索真实文献。
Dynamin-2 (DNM2) is a large GTPase belonging to the dynamin superfamily, widely expressed in eukaryotic cells and essential for membrane fission and remodeling processes. It plays a critical role in clathrin-mediated endocytosis, intracellular vesicle trafficking, and organelle maintenance, including mitochondrial division. Unlike dynamin-1. which is neuron-specific, DNM2 is ubiquitously expressed and regulates diverse cellular functions such as cytoskeletal dynamics, cell migration, and receptor signaling. Mutations in the *DNM2* gene are linked to human diseases, including centronuclear myopathy (CNM) and Charcot-Marie-Tooth neuropathy (CMT), highlighting its physiological importance.
DNM2 antibodies are immunological tools designed to detect and study the expression, localization, and functional interactions of dynamin-2 in research. These antibodies, often developed in rabbit or mouse hosts, target specific epitopes within DNM2 domains (e.g., GTPase, pleckstrin homology, or proline-rich domains). They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate DNM2’s role in cellular processes or disease mechanisms. Specific DNM2 antibodies can distinguish between isoforms or post-translationally modified forms, aiding studies on phosphorylation or disease-associated mutations. Validation across species (human, mouse, rat) ensures versatility in experimental models. Research applications range from analyzing membrane trafficking defects in genetic disorders to exploring DNM2’s involvement in cancer cell invasiveness or viral entry pathways. Proper antibody selection and validation are critical, as DNM2 shares structural homology with other dynamin family members.
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