| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HINT2 |
| Uniprot No | Q9BX68 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-163aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSVAATGVR GGQVRGAAGV TDGNEVAKAQ QATPGGAAPT IFSRILDKSL PADILYEDQQ CLVFRDVAPQ APVHFLVIPK KPIPRISQAE EEDQQLLGHL LLVAKQTAKA EGLGDGYRLV INDGKLGAQS VYHLHIHVLG GRQLQWPPG |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与HINT2重组蛋白相关的3篇文献示例(注：文献信息为模拟生成，实际引用需核实)：
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1. **文献名称**：*"Cloning, Expression, and Functional Characterization of Human HINT2 Protein in Mitochondrial Apoptosis"*
**作者**：Chen L., et al.
**摘要**：该研究成功在大肠杆菌系统中重组表达人源HINT2蛋白，并验证其在线粒体依赖性细胞凋亡中的作用，发现HINT2通过与细胞色素c的相互作用调控Caspase激活通路。
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2. **文献名称**：*"Structural Insights into HINT2 Recombinant Protein: Implications for Nucleotide Hydrolase Activity"*
**作者**：Müller R., et al.
**摘要**：通过X射线晶体学解析HINT2重组蛋白的三维结构，揭示其催化中心的保守性特征，并证明其在三磷酸核苷水解反应中的底物特异性，为靶向药物设计提供结构基础。
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3. **文献名称**：*"HINT2 Recombinant Protein Suppresses Hepatocellular Carcinoma Progression via AMPK/mTOR Signaling"*
**作者**：Wang Y., et al.
**摘要**：研究利用哺乳动物细胞表达系统制备HINT2重组蛋白，证实其通过激活AMPK通路抑制肝癌细胞增殖，并在小鼠模型中降低肿瘤负荷，提示其潜在治疗价值。
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如需具体文献，建议通过PubMed或Web of Science以“HINT2 recombinant protein”“HINT2 mitochondrial function”为关键词检索近年研究。
HINT2 (Histidine Triad Nucleotide-Binding Protein 2) is a mitochondrial protein belonging to the histidine triad (HIT) superfamily, characterized by a conserved His-X-His-X-X-His motif. This 18 kDa protein is encoded by the *HINT2* gene and primarily localizes to the mitochondrial intermembrane space. It shares structural homology with HINT1. a cytosolic/nuclear tumor suppressor, but exhibits distinct functional roles tied to mitochondrial physiology.
HINT2 is implicated in regulating apoptosis and mitochondrial integrity. Studies suggest it interacts with adenine nucleotides and may modulate mitochondrial permeability transition pores (mPTP), influencing cell death pathways. Its enzymatic activity, potentially involving nucleotide hydrolysis or protein adenylylation, remains under investigation. HINT2 expression is tissue-specific, with high levels in liver, kidney, and testes, correlating with metabolic and oxidative stress responses.
Dysregulation of HINT2 has been linked to diseases such as hepatocellular carcinoma, neurodegenerative disorders, and diabetes. For instance, downregulation in liver cancer correlates with enhanced cell proliferation, while its overexpression promotes apoptosis. Recombinant HINT2 proteins are typically produced in *E. coli* or eukaryotic systems using affinity tags (e.g., His-tag) for purification. These tools enable structural studies (e.g., crystallography) and functional assays to dissect its role in mitochondrial dynamics, energy metabolism, and disease mechanisms. Current research focuses on clarifying its biochemical activity, interaction partners, and therapeutic potential as a target for mitochondrial dysfunction-related pathologies.
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