| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | LAMPB; LAMP2; LAMP-2; LGP-96; LGP110 |
| Entrez GeneID | 3920 |
| clone | 8E2F2 |
| WB Predicted band size | 45kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant fragment of human CD107b (AA: extra 29-168) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于CD107b抗体的参考文献及其摘要概括：
1. **"CD107b (LAMP-1) as a marker of cytotoxic granule exocytosis in human cytotoxic T cells"**
- **作者**: Alter, G. et al.
- **摘要**: 该研究验证了CD107b作为细胞毒性T细胞(CTL)脱颗粒标志物的应用，通过流式细胞术检测其表面表达，证明其与细胞毒性功能相关，可用于评估免疫应答效果。
2. **"Lysosomal-associated membrane protein 1 (LAMP-1/CD107b) is a novel activation antigen of human platelets"**
- **作者**: Kojima, H. et al.
- **摘要**: 研究发现CD107b在血小板激活过程中表达上调，揭示了其在血小板溶酶体释放和炎症反应中的潜在作用，为血栓性疾病研究提供了新靶点。
3. **"CD107b is a marker of CD8+ T cell activation and degranulation in response to viral infections"**
- **作者**: Betts, M.R. et al.
- **摘要**: 通过分析HIV感染者外周血样本，证明CD107b表面表达可有效反映CD8+ T细胞的脱颗粒活化和抗病毒能力，为评估T细胞功能提供了可靠工具。
4. **"LAMP-1 chimeric antibody enhances antitumor efficacy by redirecting T cells to lysosomal compartments"**
- **作者**: Chen, X. et al.
- **摘要**: 该研究开发了一种靶向CD107b/LAMP-1的嵌合抗体，通过引导T细胞识别肿瘤细胞溶酶体，增强肿瘤杀伤效果，为癌症免疫治疗提供了新策略。
这些文献涵盖了CD107b在免疫细胞功能评估、疾病机制研究及治疗开发中的应用。
CD107b, also known as lysosome-associated membrane protein-2 (LAMP-2), is a member of the LAMP family of glycoproteins predominantly localized to lysosomal and endosomal membranes. Structurally, CD107b is a heavily glycosylated type-1 transmembrane protein, characterized by a luminal domain with conserved cysteines, a transmembrane region, and a short cytoplasmic tail. Alongside CD107a (LAMP-1), CD107b plays a role in maintaining lysosomal integrity, vesicular trafficking, and cellular waste processing. However, CD107b exhibits distinct expression patterns and functional roles. For example, it is more selectively involved in antigen presentation pathways and autophagy compared to CD107a.
In immunology, surface exposure of CD107b serves as a marker for degranulation in cytotoxic T cells and natural killer (NK) cells. During immune activation, lysosomal membranes fuse with the plasma membrane to release cytotoxic granules, temporarily displaying CD107b on the cell surface. Antibodies targeting CD107b are thus widely used in flow cytometry to quantify degranulation activity, providing insights into cytotoxic lymphocyte function in infections, cancers, or immunotherapies.
Mutations in the LAMP-2 gene are linked to rare lysosomal storage disorders, such as Danon disease, underscoring its biological significance. CD107b antibodies also aid in studying lysosomal dynamics, autophagy defects, and cellular stress responses in neurodegenerative or inflammatory diseases. Their specificity and utility in both research and clinical diagnostics highlight CD107b as a critical tool for investigating lysosomal biology and immune cell functionality.
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