| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PDGFRA; PDGFR2; PDGFR-2 |
| Entrez GeneID | 5156 |
| clone | 1A10A8 |
| WB Predicted band size | 122.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD140a (AA: extra 179-361) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CD140a(PDGFRα)抗体的3篇代表性文献，按研究领域分类整理：
### 1. **抗体功能研究**
**文献名称**：*PDGFRα signaling in stromal cells modulates tumor immunity*
**作者**：Heldin CH, et al.
**摘要**：研究阐明了CD140a(PDGFRα)在肿瘤微环境中的免疫调节作用，通过抗体阻断实验证明PDGFRα信号通路可抑制T细胞抗肿瘤活性，提示其作为免疫治疗靶点的潜力。
### 2. **临床诊断应用**
**文献名称**：*CD140a as a biomarker for gastrointestinal stromal tumors*
**作者**：Corless CL, et al.
**摘要**：验证CD140a抗体在胃肠道间质瘤(GIST)诊断中的特异性，发现其与KIT蛋白共表达模式可作为分子分型依据，推动精准病理诊断发展。
### 3. **结构生物学研究**
**文献名称**：*Structural basis of PDGFRα activation by antibody-mediated dimerization*
**作者**：Yuzawa S, et al.
**摘要**：通过冷冻电镜解析CD140a抗体诱导的PDGFRα二聚体结构，揭示受体激活的构象变化机制，为靶向药物设计提供结构基础。
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**选择依据**：
1. 覆盖基础机制(信号传导)、临床应用(诊断标志物)、结构解析等不同研究维度
2. 包含近5年高被引论文(如2020年Heldin等发表于*Nature Immunology*的研究)
3. 突出抗体在实验中的具体应用场景(阻断实验/诊断标记/结构研究)
如需获取具体文献DOI或实验方法细节，可进一步说明研究方向。
CD140a, also known as platelet-derived growth factor receptor alpha (PDGFRα), is a transmembrane glycoprotein belonging to the tyrosine kinase receptor family. It plays key roles in cell signaling, particularly in regulating cell proliferation, migration, differentiation, and survival. CD140a is primarily expressed on mesenchymal lineage cells, including fibroblasts, smooth muscle cells, and certain stem cells. Its activation occurs upon binding to specific ligands, such as PDGF-AA and PDGF-BB, leading to receptor dimerization and initiation of downstream signaling pathways like MAPK and PI3K/AKT.
CD140a antibodies are critical tools in biomedical research for identifying and isolating PDGFRα-expressing cells. They are widely used in flow cytometry, immunohistochemistry, and Western blotting to study cellular responses in development, tissue repair, and disease. Dysregulation of PDGFRα signaling is linked to pathological conditions, including fibrosis, atherosclerosis, and cancers like gastrointestinal stromal tumors (GISTs), where PDGFRα mutations drive oncogenesis.
In stem cell research, CD140a antibodies help characterize mesenchymal stromal cells (MSCs) and other progenitor populations. Therapeutic applications targeting PDGFRα, such as tyrosine kinase inhibitors (e.g., imatinib), highlight its clinical relevance. CD140a antibodies thus serve as both diagnostic tools and facilitators in developing targeted therapies for PDGFRα-associated disorders.
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