| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HLA-DOB |
| Uniprot No | P13765 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-224aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSGTDSPED FVIQAKADCY FTNGTEKVQF VVRFIFNLEE YVRFDSDVGM FVALTKLGQP DAEQWNSRLD LLERSRQAVD GVCRHNYRLG APFTVGRKVQ PEVTVYPERT PLLHQHNLLH CSVTGFYPGD IKIKWFLNGQ EERAGVMSTG PIRNGDWTFQ TVVMLEMTPE LGHVYTCLVD HSSLLSPVSV EWRAQSEYSW RK |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HLA-DOB重组蛋白的3篇参考文献示例(注：文献为模拟示例，实际引用请核实原文)：
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1. **文献名称**：*Expression and Purification of Recombinant HLA-DOB Protein in Mammalian Cells*
**作者**：Smith A, et al.
**摘要**：研究报道了在HEK293细胞中成功表达并纯化HLA-DOB重组蛋白，通过质谱和Western blot验证其正确折叠，为后续功能研究提供工具。
2. **文献名称**：*Structural Insights into HLA-DOB and Its Role in Peptide Loading Complex Assembly*
**作者**：Johnson R, et al.
**摘要**：利用重组HLA-DOB蛋白解析其晶体结构，揭示其与分子伴侣DM的相互作用机制，阐明其在抗原肽装载中的调控作用。
3. **文献名称**：*HLA-DOB Polymorphisms Modulate Immune Response in Autoimmune Diseases*
**作者**：Chen L, et al.
**摘要**：通过重组HLA-DOB蛋白体外实验，发现特定基因多态性影响其与T细胞受体的结合能力，提示其与类风湿性关节炎的潜在关联。
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建议通过PubMed或Google Scholar以关键词“HLA-DOB recombinant”或“HLA-DOB protein function”检索最新文献获取准确信息。
HLA-DOB, a member of the human leukocyte antigen (HLA) class II family, is a key protein involved in antigen presentation and immune regulation. Encoded within the HLA-DO gene cluster on chromosome 6. it forms a heterodimer with HLA-DM to modulate peptide loading onto MHC class II molecules. Unlike classical MHC II proteins (e.g., HLA-DR, -DQ, -DP), HLA-DOB lacks direct antigen-presenting activity but acts as a chaperone-like regulator. It fine-tunes HLA-DM function, which catalyzes the exchange of invariant chain-derived peptides for antigenic peptides in late endosomal compartments, ensuring optimal peptide-MHC II complexes for CD4+ T-cell recognition.
Recombinant HLA-DOB protein is typically produced using mammalian or insect cell expression systems to preserve post-translational modifications and structural integrity. Its production enables functional studies, such as elucidating interactions with HLA-DM or characterizing mutations linked to immune dysregulation. Researchers employ techniques like co-immunoprecipitation, surface plasmon resonance, or X-ray crystallography to investigate its regulatory role in autoimmune diseases, infections, or cancer immunotherapy. Polymorphisms in HLA-DOB have been associated with conditions like type 1 diabetes and hepatitis B persistence, highlighting its clinical relevance. Additionally, recombinant HLA-DOB serves as a tool for developing HLA-DM/DO-targeted therapies aimed at modulating antigen presentation in immune disorders. Its study continues to advance understanding of adaptive immunity and precision medicine strategies.
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