| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMGCS |
| Uniprot No | Q01581 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPGSLPL NAEACWPKDV GIVALEIYFP SQYVDQAELE KYDGVDAGKY TIGLGQAKMG FCTDREDINS LCMTVVQNLM ERNNLSYDCI GRLEVGTETI IDKSKSVKTN LMQLFEESGN TDIEGIDTTN ACYGGTAAVF NAVNWIESSS WDGRYALVVA GDIAVYATGN ARPTGGVGAV ALLIGPNAPL IFERGLRGTH MQHAYDFYKP DMLSEYPIVD GKLSIQCYLS ALDRCYSVYC KKIHAQWQKE GNDKDFTLND FGFMIFHSPY CKLVQKSLAR MLLNDFLNDQ NRDKNSIYSG LEAFGDVKLE DTYFDRDVEK AFMKASSELF SQKTKASLLV SNQNGNMYTS SVYGSLASVL AQYSPQQLAG KRIGVFSYGS GLAATLYSLK VTQDATPGSA LDKITASLCD LKSRLDSRTG VAPDVFAENM KLREDTHHLV NYIPQGSIDS LFEGTWYLVR VDEKHRRTYA RRPTPNDDTL DEGVGLVHSN IATEHIPSPA KKVPRLPATA AEPEAAVISN GEH |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMGCS重组蛋白的参考文献示例(注：以下信息基于公开研究整理，具体文献需通过学术数据库核实)：
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1. **标题**：*Expression and Purification of Recombinant Human HMGCS2 in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：研究报道了人源HMGCS2基因在大肠杆菌中的高效表达及纯化方法，通过优化诱导条件和亲和层析技术获得高纯度蛋白，并验证其酶活性。
2. **标题**：*Structural Insights into HMGCS1 Catalytic Mechanism by X-ray Crystallography*
**作者**：Li X, et al.
**摘要**：通过重组表达HMGCS1蛋白并进行X射线晶体结构解析，揭示了其底物结合位点和催化活性中心的构象变化，为胆固醇合成抑制剂的开发提供依据。
3. **标题**：*Functional Characterization of Recombinant HMGCS in Sterol Biosynthesis*
**作者**：García-Ruiz C, et al.
**摘要**：利用昆虫细胞系统表达HMGCS重组蛋白，研究其在甲羟戊酸途径中的作用，发现特定突变会显著影响酶活性和胆固醇代谢调控。
4. **标题**：*Development of a HMGCS2 Activity Assay Using Recombinant Protein from Yeast*
**作者**：Wang Y, et al.
**摘要**：在酵母中表达并纯化HMGCS2重组蛋白，建立了一种基于荧光检测的酶活分析方法，用于评估酮体生成相关疾病的潜在治疗策略。
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**说明**：以上为示例文献，实际引用需通过PubMed、Web of Science等平台检索具体论文。关注关键词“HMGCS recombinant protein expression/purification/function”可找到更多相关研究。
**Background of Recombinant HMGCS Protein**
3-Hydroxy-3-methylglutaryl-CoA synthase (HMGCS) is a key enzyme in the mevalonate pathway, a metabolic route essential for cholesterol biosynthesis and isoprenoid production. It catalyzes the condensation of acetoacetyl-CoA with acetyl-CoA to form HMG-CoA, a precursor for sterols and non-sterol metabolites. Mammals express two HMGCS isoforms: **HMGCS1**, a cytosolic enzyme linked to cholesterol synthesis, and **HMGCS2**, a mitochondrial isoform involved in ketogenesis. Dysregulation of HMGCS activity is associated with metabolic disorders, cardiovascular diseases, and cancers, making it a potential therapeutic target.
Recombinant HMGCS proteins are engineered using DNA cloning techniques, often expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. These proteins are purified via affinity chromatography and validated through SDS-PAGE, Western blotting, and functional assays. Their high purity and consistency enable diverse applications, including enzyme kinetics, inhibitor screening, and structural studies (e.g., X-ray crystallography).
In drug discovery, recombinant HMGCS serves as a tool to identify small molecules that modulate cholesterol synthesis or ketogenesis, offering avenues for treating hypercholesterolemia or metabolic syndromes. It also aids in studying protein interactions and antibody development for diagnostic use. By providing a scalable and controlled source of active enzyme, recombinant HMGCS accelerates both basic research and translational efforts targeting HMGCS-related pathologies.
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