| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMGB2 |
| Uniprot No | P26583 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-209aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHTGSMGKGDPNKPRGKMSSYAFFVQTCREEH KKKHPDSSVNFAEFSKKCSERWKTMSAKEKSKFEDMAKSDKARYDREMKN YVPPKGDKKGKKKDPNAPKRPPSAFFLFCSEHRPKIKSEHPGLSIGDTAK KLGEMWSEQSAKDKQPYEQKAAKLKEKYEKDIAAYRAKGKSEAGKKGPGR PTGSKKKNEPEDEEEEEEEEDEDEEEEDEDEE |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMGB2重组蛋白的3篇参考文献示例(内容基于学术研究主题，非真实文献)：
1. **《Recombinant HMGB2 Protein Expression and Purification in E. coli》**
- **作者**: Zhang L, et al.
- **摘要**: 该研究报道了利用大肠杆菌系统高效表达和纯化重组HMGB2蛋白的方法，通过His标签亲和层析技术获得高纯度蛋白，并验证其在体外结合DNA的能力，为后续功能研究提供基础材料。
2. **《HMGB2 Promotes Tumor Metastasis via Interaction with RAGE in Colorectal Cancer》**
- **作者**: Wang Y, et al.
- **摘要**: 研究发现重组HMGB2蛋白通过与细胞表面受体RAGE结合，激活NF-κB信号通路，增强结直肠癌细胞迁移和侵袭，提示HMGB2可能成为癌症治疗的潜在靶点。
3. **《Role of HMGB2 in Maintaining Pluripotency of Embryonic Stem Cells》**
- **作者**: Tanaka S, et al.
- **摘要**: 通过体外实验证明，重组HMGB2蛋白通过调控Oct4和Nanog等干性基因的表达，维持胚胎干细胞的多能性，并揭示其通过染色质结构重塑影响基因转录的分子机制。
4. **《HMGB2 Facilitates DNA Damage Repair through Chromatin Remodeling》**
- **作者**: Chen H, et al.
- **摘要**: 利用重组HMGB2蛋白进行体外实验，发现其通过促进染色质松弛，加速DNA损伤位点的修复蛋白募集，从而提高细胞对电离辐射的抗性。
High-mobility group box 2 (HMGB2) is a non-histone chromosomal protein belonging to the HMGB family, which is evolutionarily conserved and widely expressed in eukaryotic cells. Structurally, HMGB2 contains two DNA-binding HMG box domains and a negatively charged C-terminal tail, enabling its interaction with DNA and other proteins. It plays versatile roles in chromatin remodeling, transcriptional regulation, DNA repair, and cellular differentiation by modulating DNA flexibility, nucleosome positioning, and transcription factor accessibility. Notably, HMGB2 is highly expressed in embryonic tissues and stem cells but downregulated in most differentiated somatic cells, suggesting its importance in maintaining pluripotency and tissue development.
Recombinant HMGB2 protein is engineered through molecular cloning and heterologous expression systems (e.g., E. coli or mammalian cells) to produce purified, biologically active forms for research and therapeutic applications. Its recombinant version retains DNA-binding properties and functional motifs, allowing in vitro studies of its interactions with nucleic acids, histones, or signaling molecules like Toll-like receptors (TLRs). Researchers utilize recombinant HMGB2 to investigate its dual roles in physiological processes (e.g., spermatogenesis, neural development) and pathological conditions, including cancer progression, inflammatory diseases, and age-related disorders. In cancer, HMGB2 overexpression correlates with tumor invasiveness and poor prognosis, while in inflammation, it may act as a damage-associated molecular pattern (DAMP) molecule to trigger immune responses. Challenges remain in fully elucidating its context-dependent mechanisms and post-translational modifications. Current studies focus on harnessing recombinant HMGB2 for regenerative medicine, gene editing tools, or as a therapeutic target for precision oncology.
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