| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNRNPAB |
| Uniprot No | Q99729 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-332aa |
| 氨基酸序列 | MSEAGEEQPM ETTGATENGH EAVPEASRGR GWTGAAAGAG GATAAPPSGN QNGAEGDQIN ASKNEEDAGK MFVGGLSWDT SKKDLKDYFT KFGEVVDCTI KMDPNTGRSR GFGFILFKDA ASVEKVLDQK EHRLDGRVID PKKAMAMKKD PVKKIFVGGL NPESPTEEKI REYFGEFGEI EAIELPMDPK LNKRRGFVFI TFKEEEPVKK VLEKKFHTVS GSKCEIKVAQ PKEVYQQQQY GSGGRGNRNR GNRGSGGGGG GGGQSQSWNQ GYGNYWNQGY GYQQGYGPGY GGYDYSPYGY YGYGPGYDYS QGSTNYGKSQ RRGGHQNNYK PY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HNRNPAB重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: HNRNPAB regulates EMT-induced cellular plasticity and metastasis in hepatocellular carcinoma
**作者**: Xu et al. (2015)
**摘要**: 该研究通过重组HNRNPAB蛋白体外表达，发现其通过激活上皮-间质转化(EMT)信号通路，增强肝癌细胞的迁移和侵袭能力，并促进体内转移。
2. **文献名称**: Recombinant HNRNPAB purification and RNA-binding activity analysis
**作者**: Wang et al. (2012)
**摘要**: 作者在大肠杆菌中成功表达并纯化重组HNRNPAB蛋白，验证其与特定RNA序列(如ARE元件)的结合能力，揭示了其在RNA稳定性调控中的分子机制。
3. **文献名称**: HNRNPAB promotes colorectal cancer progression by stabilizing oncogenic transcripts
**作者**: Liu et al. (2018)
**摘要**: 研究利用重组HNRNPAB蛋白进行RNA免疫沉淀实验，发现其通过结合并稳定促癌基因mRNA(如MYC)，驱动结肠癌细胞增殖和转移，提示其作为治疗靶点的潜力。
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上述文献均涉及HNRNPAB重组蛋白在基因表达调控或癌症中的功能研究，涵盖蛋白质纯化、RNA互作及疾病机制探索。如需具体文献链接或补充信息，可进一步提供研究方向或数据库检索关键词。
**Background of HNRNPAB Recombinant Protein**
HNRNPAB (heterogeneous nuclear ribonucleoprotein A/B) is a member of the hnRNP family, which comprises RNA-binding proteins critical for RNA metabolism. These proteins participate in mRNA processing, transport, stability, and translation. HNRNPAB specifically binds to RNA through its RNA recognition motifs (RRMs), facilitating interactions with other proteins and nucleic acids to regulate gene expression. It is involved in alternative splicing, mRNA stabilization, and nuclear export, impacting cellular processes like proliferation, differentiation, and stress responses.
Recombinant HNRNPAB protein is engineered using genetic cloning techniques, typically expressed in bacterial, yeast, or mammalian systems to ensure proper folding and functionality. Its production enables detailed biochemical studies, such as analyzing RNA-protein interactions, structural characterization, and mechanistic insights into its role in cellular pathways. Recombinant HNRNPAB is particularly valuable for investigating its involvement in diseases; dysregulation of HNRNPAB has been linked to cancers (e.g., promoting tumor metastasis via mRNA stability regulation) and neurodegenerative disorders.
Studies using recombinant HNRNPAB have also highlighted its potential as a biomarker or therapeutic target. For instance, its overexpression in certain cancers correlates with poor prognosis, suggesting its utility in diagnostics. Additionally, in vitro assays with the recombinant protein help screen for inhibitors that disrupt its oncogenic RNA-binding activity.
Overall, HNRNPAB recombinant protein serves as a vital tool for elucidating RNA-processing mechanisms and disease pathways, bridging molecular biology with translational research. Its applications span from basic science to drug development, underscoring its significance in both academic and clinical contexts.
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