| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMGN3 |
| Uniprot No | Q15651-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-77aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPKRKSP ENTEGKDGSK VTKQEPTRRS ARLSAKPAPP KPEPKPRKTS AKKEPGAKIS RGAKGKKEEK QEAGKEGTEN |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMGN3重组蛋白的3篇示例文献(内容为假设性概括，仅供参考)：
1. **文献名称**: *"Recombinant HMGN3 Protein Enhances Chromatin Accessibility and Promotes Transcriptional Activation"*
**作者**: Smith J, et al.
**摘要**: 研究通过大肠杆菌系统成功表达并纯化了HMGN3重组蛋白，证实其能够结合核小体并增加染色质可及性，促进特定基因(如细胞周期相关基因)的转录激活。
2. **文献名称**: *"Structural and Functional Analysis of HMGN3 in DNA Damage Response"*
**作者**: Chen L, et al.
**摘要**: 利用重组HMGN3蛋白解析了其与DNA损伤修复复合物的相互作用机制，发现HMGN3通过调控组蛋白修饰(如H3K4甲基化)增强细胞对电离辐射的应答能力。
3. **文献名称**: *"HMGN3 Recombinant Protein Suppresses Tumor Growth in Hepatocellular Carcinoma Models"*
**作者**: Wang Y, et al.
**摘要**: 在肝癌细胞系和小鼠模型中，外源性添加HMGN3重组蛋白显著抑制肿瘤增殖，机制涉及下调致癌基因MYC并激活抑癌通路p53.
---
**注**：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science检索真实文献。如需具体文献指导，请提供更详细的研究方向(如疾病模型或分子机制)。
HMGN3 (High Mobility Group Nucleosome-Binding Protein 3) is a member of the HMGN protein family, which plays a critical role in modulating chromatin structure and regulating gene expression. These proteins bind to nucleosomes—the basic units of chromatin—through a conserved nucleosome-binding domain, enhancing chromatin accessibility for transcriptional machinery. HMGN3. specifically, is involved in fine-tuning cellular processes such as differentiation, proliferation, and response to DNA damage. Studies suggest it interacts with transcription factors and epigenetic modifiers to influence cell type-specific gene programs, particularly in tissues like the liver, pancreas, and brain. Dysregulation of HMGN3 has been linked to diseases, including certain cancers, where altered expression may contribute to tumor progression or suppression depending on context.
Recombinant HMGN3 protein is engineered using expression systems (e.g., E. coli or mammalian cells) to produce a purified, functional form of the protein for research. This tool enables scientists to study HMGN3’s biochemical properties, such as its binding affinity to nucleosomes or DNA, and its role in chromatin remodeling. Researchers also use it to explore interactions with partner proteins or to assess its impact on gene regulation in vitro or in cellular models. For instance, recombinant HMGN3 has been utilized to investigate its dual role in cancer—either promoting oncogenesis by activating proliferation-related genes or suppressing tumors by maintaining genomic stability.
The development of recombinant HMGN3 has advanced our understanding of its therapeutic potential. It serves as a reagent for drug screening assays targeting chromatin-related pathways and offers insights into designing epigenetic therapies. However, its pleiotropic effects underscore the need for context-specific studies to harness its functions safely. Ongoing research continues to unravel HMGN3’s complexities in health and disease, highlighting its significance in both basic and translational biology.
×
