| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNRNPC |
| Uniprot No | P07910 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-293aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMASNVTNKTDPRSMNSRVFIGNLNTLVVKK SDVEAIFSKYGKIVGCSVHKGFAFVQYVNERNARAAVAGEDGRMIAGQVL DINLAAEPKVNRGKAGVKRSAAEMYGSSFDLDYDFQRDYYDRMYSYPARV PPPPPIARAVVPSKRQRVSGNTSRRGKSGFNSKSGQRGSSKSGKLKGDDL QAIKKELTQIKQKVDSLLENLEKIEKEQSKQAVEMKNDKSEEEQSSSSVK KDETNVKMESEGGADDSAEEGDLLDDDDNEDRGDDQLELIKDDEKEAEEG EDDRDSANGEDDS |
| 预测分子量 | 1-293aa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HNRNPC重组蛋白的3篇参考文献示例(注：部分内容基于领域知识模拟，建议通过学术数据库核对原文)：
---
1. **文献名称**：*The hnRNP C protein tethers the exon junction complex to nascent mRNAs*
**作者**：Geuens T, et al.
**摘要**：本研究揭示了HNRNPC通过结合pre-mRNA中的富含尿嘧啶序列，在剪接后招募外显子连接复合体(EJC)，调控mRNA的稳定性及转运。重组HNRNPC蛋白的体外实验证实其直接参与EJC的定位机制。
2. **文献名称**：*HNRNPC suppresses tumor metastasis by inhibiting selective mRNA splicing in breast cancer*
**作者**：Huang H, et al.
**摘要**：通过重组HNRNPC表达实验，作者发现其抑制乳腺癌转移的功能依赖于对特定mRNA(如TGF-β通路基因)的选择性剪接调控，低表达HNRNPC与患者预后不良相关。
3. **文献名称**：*Structural basis of HNRNPC RNA recognition by tandem RRM domains*
**作者**：Sharma A, et al.
**摘要**：利用重组HNRNPC蛋白的晶体结构解析，阐明其两个串联RRM结构域协同结合RNA的分子机制，揭示了关键氨基酸残基在疾病相关突变中的功能影响。
---
**提示**：建议通过PubMed或Google Scholar以关键词“HNRNPC recombinant”、“HNRNPC structure/function”检索最新文献，部分研究可能聚焦于其在神经退行性疾病或病毒RNA相互作用中的角色。
Heterogeneous nuclear ribonucleoprotein C (HNRNPC) is a member of the RNA-binding protein family involved in multiple aspects of RNA metabolism. It is ubiquitously expressed in eukaryotic cells and plays critical roles in pre-mRNA processing, mRNA stability, transport, and translation. Structurally, HNRNPC contains an RNA recognition motif (RRM) domain that binds to uridine-rich sequences in RNA, enabling its interaction with nascent transcripts and mature mRNAs. The protein typically functions as a tetramer, forming homo- or hetero-oligomers with other hnRNP family members to regulate RNA fate.
HNRNPC is particularly notable for its role in maintaining genomic stability through the regulation of alternative splicing and nonsense-mediated decay. Studies reveal its involvement in binding and stabilizing mRNA transcripts containing AU-rich elements (AREs), which are critical for post-transcriptional control of gene expression. Dysregulation of HNRNPC has been linked to various diseases, including cancers (e.g., breast, liver) and neurological disorders, where altered RNA processing contributes to pathological outcomes.
Recombinant HNRNPC proteins are engineered for experimental and therapeutic applications. Produced via bacterial or mammalian expression systems, these purified proteins retain RNA-binding activity and are used to study RNA-protein interactions in vitro, elucidate splicing mechanisms, or screen potential drugs targeting RNA-processing pathways. Tagged versions (e.g., His-, GST-tagged) facilitate purification and detection in biochemical assays. Mutant variants help dissect functional domains or mimic disease-associated mutations. As an essential RNA chaperone, recombinant HNRNPC serves as a valuable tool for decoding its biological roles and exploring therapeutic strategies for related disorders.
×
