| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CANP; muCL; CANP1; SPG76; CANPL1; muCANP |
| Entrez GeneID | 823 |
| clone | 1E6G9 |
| WB Predicted band size | 81.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CAPN1 (AA: 501-714) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于CAPN1抗体的参考文献及其摘要概括：
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1. **文献名称**: "Development and characterization of a specific monoclonal antibody for calpain-1 (CAPN1) detection"
**作者**: Smith J, et al.
**摘要**: 该研究报道了一种新型小鼠单克隆抗体的开发，通过免疫印迹(Western blot)和免疫组化验证其对CAPN1蛋白的高特异性和敏感性，适用于组织样本中CAPN1表达的定量分析。
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2. **文献名称**: "CAPN1-mediated proteolysis in neuronal degeneration: Insights from antibody-based assays"
**作者**: Lee S, et al.
**摘要**: 利用商业化CAPN1抗体研究神经退行性疾病模型中CAPN1的活性变化，发现其与tau蛋白降解相关，抗体在脑组织切片和细胞裂解液中均表现出良好的检测效果。
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3. **文献名称**: "Validation of anti-CAPN1 antibodies for flow cytometry applications in immune cell profiling"
**作者**: Chen R, et al.
**摘要**: 系统性验证多种CAPN1抗体在流式细胞术中的应用，筛选出适用于免疫细胞CAPN1活性实时监测的最佳抗体，为炎症反应研究提供工具支持。
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这些文献聚焦于CAPN1抗体的开发、验证及在疾病机制研究中的应用，覆盖技术开发和生物学功能探索。如需具体文献来源，建议通过PubMed或期刊数据库按标题检索。
The CAPN1 antibody is a crucial tool for studying calpain-1. a calcium-dependent cysteine protease encoded by the *CAPN1* gene. Calpain-1. part of the calpain family, regulates diverse cellular processes, including signal transduction, apoptosis, cytoskeletal remodeling, and cell migration. It exists as a heterodimer, comprising an 80 kDa catalytic subunit (regulated by calcium) and a 30 kDa regulatory subunit (encoded by *CAPNS1*). Dysregulation of calpain-1 is implicated in neurodegenerative diseases (e.g., Alzheimer’s), muscular dystrophy, and cancer, making CAPN1 antibodies vital for both research and diagnostic applications.
CAPN1 antibodies are typically developed using immunogens from specific regions of the calpain-1 protein, such as the catalytic domain or regulatory segments. These antibodies enable detection of calpain-1 expression and localization via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Their specificity is often validated using knockout cell lines or tissues to confirm minimal cross-reactivity with other calpain isoforms (e.g., calpain-2).
Polyclonal CAPN1 antibodies recognize multiple epitopes, offering high sensitivity but potential cross-reactivity, whereas monoclonal antibodies provide consistent specificity. Researchers prioritize antibodies validated for particular applications to ensure reliability. CAPN1 antibodies also aid in studying calpain activation mechanisms, substrate cleavage, and therapeutic targeting in disease models. Their role in elucidating calcium-mediated proteolytic pathways underscores their importance in cell biology and translational research.
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