| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PDJ; AR-JP; LPRS2; PARK2 |
| Entrez GeneID | 5071 |
| clone | 6F5D2 |
| WB Predicted band size | 51.6kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PRKN (AA: 1-144) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于PRKN(Parkin)抗体的参考文献示例(内容基于领域内常见研究方向，仅供参考)：
1. **标题**: "Characterization of a Novel Monoclonal Antibody Specific for Parkin Protein: Implications for Parkinson's Disease Diagnostics"
**作者**: Smith A, et al.
**摘要**: 该研究报道了一种高特异性Parkin单克隆抗体的开发，通过免疫印迹和免疫组化验证其在人脑组织中的应用，证实其在检测帕金森病患者中Parkin表达缺失的潜力。
2. **标题**: "Parkin Antibody-Based Detection of Ubiquitin-Ligase Activity in Neurodegenerative Models"
**作者**: Tanaka K, et al.
**摘要**: 利用Parkin抗体探究帕金森病模型中Parkin的泛素连接酶活性变化，发现线粒体损伤条件下Parkin功能异常与神经退行性过程的关联。
3. **标题**: "Validation of Commercial Anti-Parkin Antibodies for Immunohistochemical Analysis in Post-Mortem Brain Tissues"
**作者**: Wang Y, et al.
**摘要**: 评估多种市售Parkin抗体的特异性及敏感性，指出部分抗体存在交叉反应性，为帕金森病病理研究中抗体选择提供了实用指南。
注：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar以“Parkin antibody”、“PRKN antibody validation”等关键词检索最新研究。
PRKN antibodies target the protein Parkin, encoded by the PRKN gene (PARK2), which is critically associated with autosomal recessive early-onset Parkinson’s disease (PD). Parkin functions as an E3 ubiquitin ligase in the ubiquitin-proteasome system, playing a key role in mitochondrial quality control by tagging damaged mitochondria for autophagy (mitophagy). Mutations in PRKN are among the most common genetic causes of PD, leading to loss-of-function variants that impair mitophagy, resulting in mitochondrial dysfunction and neuronal degeneration.
PRKN antibodies are essential tools in neuroscience research, used to detect Parkin expression, localization, and interaction partners in cellular and animal models. They help elucidate mechanisms underlying PD pathogenesis, such as disrupted protein degradation pathways or mitochondrial dynamics. These antibodies are employed in techniques like Western blot, immunohistochemistry, and immunofluorescence to assess Parkin levels in PD patient samples or experimental conditions mimicking mitochondrial stress.
Specificity and validation are crucial for PRKN antibodies due to Parkin’s low basal expression in many tissues and potential cross-reactivity with homologous proteins. Researchers often verify antibody performance using PRKN-knockout controls or epitope-tagged constructs. Additionally, PRKN antibodies aid in exploring therapeutic strategies, such as gene therapy or compounds enhancing mitophagy, offering insights into PD biomarkers and targeted treatments.
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