| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | 1C5; HS1; 14-3-3 |
| Entrez GeneID | 10971 |
| clone | 5C4E7 |
| WB Predicted band size | 27.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human YWHAQ (AA: 1-245) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于YWHAQ抗体的3篇参考文献及其简要摘要：
1. **文献名称**: *14-3-3 proteins in neurological disorders*
**作者**: Aitken A, et al.
**摘要**: 该研究综述了14-3-3蛋白家族(包括YWHAQ/14-3-3θ)在神经退行性疾病中的作用，提到使用YWHAQ抗体检测脑脊液中14-3-3θ的表达水平，作为阿尔茨海默病的潜在生物标志物。
2. **文献名称**: *Interaction of 14-3-3θ with tau protein regulates microtubule stability*
**作者**: Sluchanko NN, et al.
**摘要**: 研究通过免疫共沉淀和Western blot(使用YWHAQ抗体)证实，YWHAQ与tau蛋白结合，调控微管稳定性，提示其在tau病理相关疾病(如帕金森病)中的潜在机制。
3. **文献名称**: *Proteomic analysis of 14-3-3θ-associated proteins in cancer cell signaling*
**作者**: Neal CL, et al.
**摘要**: 利用YWHAQ抗体进行免疫沉淀结合质谱分析，鉴定出多种与YWHAQ互作的癌相关蛋白(如Raf-1、BAD)，揭示其在PI3K/AKT和MAPK信号通路中的调控作用。
4. **文献名称**: *Dysregulation of 14-3-3θ in autoimmune diseases*
**作者**: Li Y, et al.
**摘要**: 通过ELISA和免疫组化(使用特异性YWHAQ抗体)发现，YWHAQ在类风湿性关节炎患者滑膜组织中异常高表达，可能通过激活NF-κB通路促进炎症反应。
以上研究均涉及YWHAQ抗体的应用，涵盖疾病机制、蛋白互作及诊断标志物探索。
**Background of YWHAQ Antibody**
The YWHAQ antibody targets the YWHAQ protein, a member of the 14-3-3 family of conserved regulatory molecules. These proteins play critical roles in signal transduction, cell cycle control, apoptosis, and stress response by binding to phosphorylated serine/threonine residues on client proteins, modulating their activity, stability, or localization. YWHAQ (also known as 14-3-3τ or theta) is ubiquitously expressed and implicated in diverse cellular processes, including cancer progression, neurodegenerative diseases, and metabolic regulation.
YWHAQ antibodies are widely used in research to investigate protein-protein interactions, post-translational modifications, and signaling pathways. They enable detection and quantification of YWHAQ in techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Dysregulation of YWHAQ has been linked to pathologies such as Alzheimer’s disease (via tau protein interactions) and cancers (e.g., breast, lung), where it may influence drug resistance or tumorigenesis.
The development of specific YWHAQ antibodies has been crucial for elucidating its mechanistic roles and potential as a therapeutic target. Recent studies also explore its involvement in immune responses and viral infections, highlighting its multifaceted biological significance. Validated antibodies with high specificity remain essential tools for advancing both basic and translational research in these areas.
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