| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CKMM; M-CK; CPK-M |
| Entrez GeneID | 1158 |
| clone | 1D10F2 |
| WB Predicted band size | 43.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CKM (AA: 1-381) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CKM(肌酸激酶M型)抗体的3篇示例文献(注：内容为示例性概括，实际文献需通过学术数据库验证)：
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1. **文献名称**: *Creatine kinase-M isoenzyme as a biomarker for acute myocardial infarction: diagnostic value in early phase*
**作者**: Apple FS, et al.
**摘要**: 研究评估了CKM抗体在ELISA检测中对急性心肌梗死(AMI)的早期诊断价值。结果显示，CKM在AMI发作后3-6小时显著升高，其敏感性和特异性优于传统心肌标志物，提示其作为早期诊断工具的潜力。
2. **文献名称**: *Immunohistochemical detection of CKM in skeletal muscle diseases: correlation with disease progression*
**作者**: Darras BT, et al.
**摘要**: 通过CKM抗体的免疫组化分析，发现杜氏肌营养不良(DMD)患者骨骼肌中CKM表达异常降低，与肌肉退化程度呈负相关。该研究支持CKM抗体在肌肉病理诊断和疾病分期中的应用。
3. **文献名称**: *Monoclonal antibody-based assay for creatine kinase-M: optimization and clinical validation*
**作者**: Wu AH, et al.
**摘要**: 开发了一种高特异性的单克隆CKM抗体检测方法，对比传统电泳法显示更高的灵敏度和重复性。该方法成功应用于临床样本，显著提高了肌肉损伤和心肌疾病的检测效率。
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**备注**：以上文献信息为示例，实际引用时请通过PubMed、Web of Science等平台检索真实文献(关键词如"Creatine kinase M antibody"、"CKM immunoassay")。若需具体文献，可提供更详细的研究方向(如疾病类型或技术方法)。
CKM antibodies target creatine kinase M-type (CKM), a key enzyme involved in cellular energy metabolism. Creatine kinase (CK) catalyzes the reversible transfer of phosphate between creatine and adenosine triphosphate (ATP), playing a critical role in maintaining energy homeostasis, particularly in tissues with high energy demands like skeletal muscle, heart, and brain. The CK system exists as dimeric isoforms: CK-MM (muscle), CK-MB (cardiac), and CK-BB (brain). CKM refers to the muscle-specific isoform encoded by the *CKM* gene, predominantly expressed in skeletal muscle and, to a lesser extent, in cardiac tissue.
CKM antibodies are widely used in research and diagnostics to detect and quantify CK-MM, aiding in the assessment of muscle damage or disease. Elevated CK-MM levels in blood are biomarkers for conditions such as muscular dystrophy, rhabdomyolysis, or myocardial injury. In research, these antibodies help study muscle regeneration, mitochondrial function, and pathologies linked to energy dysregulation. They are also employed in techniques like Western blot, immunohistochemistry, and ELISA to evaluate tissue-specific expression patterns. Additionally, CKM antibodies have applications in studying cancer, as some tumors exhibit aberrant CK expression. Their specificity for the M-subunit makes them valuable tools for distinguishing muscle-related pathologies from other CK isoform-associated disorders.
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