| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | bsd |
| Uniprot No | P0C2P0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-130aa |
| 氨基酸序列 | MPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAIVKDSDGQPTAVGIRELLPSGYVWEG |
| 预测分子量 | 20.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BSD重组蛋白的3篇参考文献示例(注：文献信息为模拟虚构，仅供参考)：
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1. **文献名称**：*Expression and Purification of Recombinant BSD Protein in E. coli for Selection Marker Applications*
**作者**：Chen L., et al.
**摘要**：本研究报道了在大肠杆菌系统中高效表达BSD(Blasticidin S脱氨酶)重组蛋白的优化方法，通过密码子优化与诱导条件调控，显著提高蛋白可溶性表达水平，并验证其作为哺乳动物细胞筛选标记的有效性。
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2. **文献名称**：*Crystal Structure Analysis of BSD Mutants with Enhanced Enzymatic Stability*
**作者**：Tanaka K., et al.
**摘要**：通过X射线晶体学解析了BSD蛋白及其突变体的三维结构，揭示了关键氨基酸残基对Blasticidin S脱氨酶活性与稳定性的影响，为设计抗性更强的新型选择标记提供结构基础。
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3. **文献名称**：*Development of a BSD-Based Bicistronic Vector for Simultaneous Gene Expression and Selection in Mammalian Cells*
**作者**：Müller R., et al.
**摘要**：构建了一种基于BSD的双顺反子载体系统，可在哺乳动物细胞中实现目标基因表达与Blasticidin抗性筛选的同步进行，显著提高转染效率并降低筛选成本。
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如需实际文献，建议通过PubMed或Web of Science检索关键词“Blasticidin S deaminase”、“recombinant BSD protein”或“BSD selection marker”。
**Background of BSD Recombinant Protein**
The BSD (Blasticidin S deaminase) recombinant protein is a key tool in molecular biology and genetic engineering, primarily recognized for its role as a selection marker in eukaryotic and prokaryotic expression systems. Originating from *Aspergillus terreus*, the *bsd* gene encodes an enzyme that inactivates Blasticidin S, a potent antibiotic derived from *Streptomyces griseochromogenes*. This antibiotic inhibits protein synthesis by interfering with peptide bond formation in both prokaryotic and eukaryotic cells.
BSD’s utility emerged in the 1990s as researchers sought versatile selection markers for stable transfection. Unlike traditional markers (e.g., neomycin resistance), BSD offers faster selection (within 3–5 days) and compatibility with diverse cell lines, including mammalian, insect, and bacterial systems. The recombinant BSD protein is typically fused to genes of interest or expressed alongside them via bicistronic vectors, enabling efficient selection of transfected cells under Blasticidin S pressure.
Its applications span gene therapy, drug development, and functional genomics. In gene editing (e.g., CRISPR/Cas9), BSD is used to co-select cells harboring correct genetic modifications. Additionally, its small size (∼16 kDa) and minimal cytotoxicity make it ideal for sensitive experiments. Challenges include optimizing expression levels to balance selection efficiency and cellular stress.
Recent advancements focus on engineering BSD variants with enhanced stability or reduced immunogenicity for *in vivo* studies. Overall, BSD recombinant protein remains a cornerstone in genetic manipulation, offering reliability and adaptability across experimental models.
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