| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HPSE |
| Uniprot No | Q9Y251 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-543aa |
| 氨基酸序列 | QDVVDLDFFTQEPLHLVSPSFLSVTIDANLATDPRFLILLGSPKLRTLAR GLSPAYLRFGGTKTDFLIFDPKKESTFEERSYWQSQVNQDICKYGSIPPD VEEKLRLEWPYQEQLLLREHYQKKFKNSTYSRSSVDVLYTFANCSGLDLI FGLNALLRTADLQWNSSNAQLLLDYCSSKGYNISWELGNEPNSFLKKADI FINGSQLGEDFIQLHKLLRKSTFKNAKLYGPDVGQPRRKTAKMLKSFLKA GGEVIDSVTWHHYYLNGRTATKEDFLNPDVLDIFISSVQKVFQVVESTRP GKKVWLGETSSAYGGGAPLLSDTFAAGFMWLDKLGLSARMGIEVVMRQVF FGAGNYHLVDENFDPLPDYWLSLLFKKLVGTKVLMASVQGSKRRKLRVYL HCTNTDNPRYKEGDLTLYAINLHNVTKYLRLPYPFSNKQVDKYLLRPLGP HGLLSKSVQLNGLTLKMVDDQTLPPLMEKPLRPGSSLGLPAFSYSFFVIR NAKVAACI |
| 预测分子量 | 59 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HPSE(Heparanase)重组蛋白的3篇参考文献示例(注：内容为示例概括，建议通过学术数据库核对真实文献)：
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1. **文献名称**: "Cloning and functional expression of human heparanase in tumor progression"
**作者**: Vlodavsky, I., et al.
**摘要**: 该研究首次报道了人源HPSE基因的克隆及重组蛋白在哺乳动物细胞中的表达，证实重组HPSE具有切割硫酸乙酰肝素多糖链的活性，并促进肿瘤细胞侵袭和血管生成。研究为HPSE在癌症转移中的作用提供了分子机制证据。
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2. **文献名称**: "Crystal structure of human heparanase reveals molecular basis for substrate recognition"
**作者**: Wu, L., et al.
**摘要**: 通过X射线晶体学解析了重组HPSE蛋白的三维结构，揭示了其底物结合域的关键氨基酸残基及催化机制，为设计特异性抑制剂提供了结构基础。研究还利用重组HPSE验证了突变体对酶活性的影响。
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3. **文献名称**: "Recombinant heparanase enhances macrophage migration and inflammatory response via syndecan-1 shedding"
**作者**: Sanderson, R.D., et al.
**摘要**: 研究利用重组HPSE蛋白处理巨噬细胞，发现其通过裂解细胞表面Syndecan-1触发炎症信号通路，促进细胞迁移和细胞因子释放。该发现揭示了HPSE在炎症性疾病中的潜在调控作用。
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建议通过PubMed或Google Scholar检索关键词“recombinant heparanase”获取最新文献。
**Background of Recombinant Heparanase (HPSE) Protein**
Heparanase (HPSE) is an endo-β-glucuronidase enzyme that specifically cleaves heparan sulfate (HS), a complex polysaccharide found in the extracellular matrix (ECM) and on cell surfaces. HS plays critical roles in regulating cellular processes such as inflammation, angiogenesis, and cell migration by interacting with growth factors, cytokines, and ECM components. HPSE-mediated cleavage of HS disrupts these interactions, releasing bioactive fragments and altering ECM integrity, which influences physiological and pathological conditions, including cancer metastasis, autoimmune diseases, and tissue remodeling.
The human HPSE gene encodes a 65 kDa latent pro-enzyme that undergoes proteolytic processing to generate a 50 kDa active heterodimer. Recombinant HPSE proteins are produced using expression systems like mammalian cells, bacteria, or insect cells, enabling controlled studies of its enzymatic activity and biological functions. Recombinant technology allows for the generation of high-purity, functional HPSE, facilitating research into its role in diseases and therapeutic targeting.
HPSE is a double-edged sword in disease contexts. While its overexpression in cancers promotes tumor invasion, angiogenesis, and metastasis by degrading ECM barriers, it also modulates immune responses and tissue repair. Inhibiting HPSE has emerged as a therapeutic strategy in oncology, driving the development of neutralizing antibodies, small-molecule inhibitors, and engineered recombinant variants. Conversely, recombinant HPSE is explored for its potential in enhancing drug delivery by degrading HS barriers in tissues or mitigating fibrosis.
Research on recombinant HPSE also extends to inflammatory disorders (e.g., inflammatory bowel disease) and metabolic conditions, where HS remodeling impacts disease progression. However, challenges remain in balancing its enzymatic activity to avoid off-target effects. Overall, recombinant HPSE serves as a vital tool for deciphering HS-dependent mechanisms and advancing targeted therapies.
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