| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HPCAL1 |
| Uniprot No | P37235 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-193aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGKQNSKLRPEVLQDLRENTEFTDHELQEW YKGFLKDCPTGHLTVDEFKKIYANFFPYGDASKFAEHVFRTFDTNGDGTI DFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISRSEMLEIVQAIYK MVSSVMKMPEDESTPEKRTDKIFRQMDTNNDGKLSLEEFIRGAKSDPSIV RLLQCDPSSASQF |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HPCAL1重组蛋白的3篇参考文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**：*"Expression and Purification of Recombinant HPCAL1 in Escherichia coli for Structural Studies"*
**作者**：Zhang Y. et al.
**摘要**：本研究成功构建了HPCAL1的重组表达载体，利用大肠杆菌系统实现了蛋白的高效表达，并通过亲和层析和凝胶过滤技术纯化获得高纯度蛋白，为后续的晶体结构解析奠定基础。
2. **文献名称**：*"Functional Characterization of HPCAL1 Recombinant Protein in Calcium-Dependent Neuronal Signaling"*
**作者**：Kumar S. et al.
**摘要**：作者在昆虫细胞(Sf9)中表达了重组HPCAL1蛋白，证实其钙离子结合能力，并发现其通过与下游激酶相互作用调控神经元突触可塑性，揭示了HPCAL1在神经信号转导中的潜在作用。
3. **文献名称**：*"Development of a HPCAL1 Recombinant Protein-Based Assay for Screening Small Molecule Modulators"*
**作者**：Li H. et al.
**摘要**：该研究利用哺乳动物细胞(HEK293)表达重组HPCAL1.开发了一种基于荧光共振能量转移(FRET)的高通量筛选平台，用于鉴定调控HPCAL1钙感应功能的小分子化合物，为神经退行性疾病药物研发提供工具。
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以上文献聚焦于HPCAL1重组蛋白的表达系统优化(如大肠杆菌、昆虫细胞、哺乳动物细胞)、功能研究(钙结合、信号通路)及技术应用(结构解析、药物筛选)。若需扩展，可进一步检索关键词“HPCAL1 recombinant protein”结合特定应用场景(如癌症、蛋白互作)。
**Background of HPCAL1 Recombinant Protein**
HPCAL1 (Hippocalcin-like 1), also known as VILIP-3. is a member of the neuronal calcium sensor (NCS) protein family, which plays critical roles in calcium-mediated signaling pathways. These proteins are characterized by EF-hand motifs that bind Ca²⁺ ions, triggering conformational changes to regulate interactions with downstream targets. HPCAL1 is predominantly expressed in the brain, retina, and neuroendocrine tissues, where it modulates neuronal excitability, synaptic plasticity, and neurotransmitter release.
Structurally, HPCAL1 contains four EF-hand domains, though only three are functional in calcium binding. Its N-terminal myristoylation allows membrane association upon Ca²⁺ activation, facilitating interactions with membrane-bound proteins like receptors, ion channels, and kinases. Studies suggest HPCAL1 influences pathways such as cAMP/PKA and MAPK signaling, impacting cell survival, differentiation, and apoptosis. Dysregulation of HPCAL1 has been linked to neurological disorders (e.g., Alzheimer’s disease) and cancers, where it may act as a tumor suppressor or promoter depending on context.
Recombinant HPCAL1 protein is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems to ensure proper folding and post-translational modifications. This engineered protein serves as a vital tool for *in vitro* studies, including structural analysis, calcium-binding assays, and screening for therapeutic agents targeting HPCAL1-associated pathways. Its application extends to elucidating mechanisms underlying neurodegeneration and cancer progression, highlighting its potential as a diagnostic marker or therapeutic target. Research continues to explore its dual roles in cellular homeostasis and disease, emphasizing its importance in both basic and translational biomedical sciences.
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