| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOPX |
| Uniprot No | Q9BPY8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-73aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMSAETA SGPTEDQVEI LEYNFNKVDK HPDSTTLCLI AAEAGLSEEE TQKWFKQRLA KWRRSEGLPS ECRSVTD |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HOPX重组蛋白的3篇代表性文献及其摘要概括：
1. **《HOPX suppresses the proliferation and tumorigenesis of gastric cancer by downregulating Wnt/β-catenin signaling》**
- 作者：Kota, S. 等
- 摘要：该研究通过重组HOPX蛋白体外处理胃癌细胞，发现其能抑制Wnt/β-catenin信号通路，减少细胞增殖和肿瘤形成，提示HOPX在胃癌中具有抑癌作用。
2. **《HOPX regulates cardiomyocyte development via chromatin remodeling complexes》**
- 作者：Chen, J. 等
- 摘要：利用重组HOPX蛋白进行功能实验，揭示其通过结合染色质重塑复合物(如NuRD)调控心脏发育相关基因表达，影响心肌细胞分化和心脏形态发生。
3. **《Epigenetic silencing of HOPX promotes cancer stem cell properties in colorectal cancer》**
- 作者：Tsubooka, N. 等
- 摘要：研究通过重组HOPX蛋白恢复其在结直肠癌细胞中的表达，发现可降低肿瘤干细胞标志物水平并抑制肿瘤球形成，表明HOPX缺失促进肿瘤干性维持。
4. **《HOPX functions as a tumour suppressor in head and neck squamous cell carcinoma》**
- 作者：Tomasek, M. 等
- 摘要：通过重组HOPX蛋白实验证明其在头颈鳞癌中通过调控细胞周期蛋白(如p21)抑制细胞周期进程，并增强化疗敏感性，提示其作为治疗靶点的潜力。
**Background of HOPX Recombinant Protein**
HOPX (Homeodomain-only protein X), also known as HOD (Homeobox-only protein), is a small, non-canonical homeodomain-containing protein that plays diverse roles in cellular differentiation, tissue development, and disease progression. Unlike typical homeobox proteins, HOPX lacks DNA-binding activity due to its truncated structure but acts as a transcriptional co-regulator by interacting with chromatin-modifying complexes (e.g., HDACs) and transcription factors (e.g., SRF). It is evolutionarily conserved and expressed in multiple tissues, including the heart, brain, and epithelial systems.
HOPX is implicated in regulating cell proliferation, apoptosis, and lineage-specific differentiation. In cardiac development, HOPX modulates cardiomyocyte maturation and heart morphogenesis. In stem cells, it maintains quiescence and prevents premature differentiation. Notably, HOPX has dual roles in cancer—acting as a tumor suppressor in certain contexts (e.g., colorectal and lung cancers) by inhibiting Wnt/β-catenin signaling, while promoting aggressiveness in others (e.g., gliomas) via epithelial-mesenchymal transition (EMT).
Recombinant HOPX protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, bioactive forms for functional studies. Its recombinant version enables researchers to explore HOPX-mediated signaling pathways, protein interactions, and therapeutic potential. Applications include *in vitro* assays (e.g., binding kinetics, gene regulation), disease modeling, and drug screening.
Overall, HOPX recombinant protein serves as a critical tool for unraveling its complex biological roles and translational relevance in regenerative medicine and oncology.
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