| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOMER2 |
| Uniprot No | Q9NSB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 氨基酸序列 | MGEQPIFTTRAHVFQIDPNTKKNWMPASKQAVTVSYFYDVTRNSYRIISVDGAKVIINSTITPNMTFTKTSQKFGQWADSRANTVFGLGFSSEQQLTKFAEKFQEVKEAAKIAKDKTQEKIETSSNHSQESGRETPSSTQASSVNGTDDEKASHAGPANTHLKSENDKLKIALTQSAANVKKWEIELQTLRESNARLTTALQESAASVEQWKRQFSICRDENDRLRNKIDELEEQCSEINREKEKNTQLKRRIEELEAELREKETELKDLRKQSEIIPQLMSECEYVSEKLEAAERDNQNLEDKVRSLKTDIEESKYRQRHLKVELKSFLEVLDGKIDDLHDFRRGLSKLGTDN |
| 预测分子量 | 48.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HOMER2重组蛋白的3篇参考文献，涵盖其结构、功能及在疾病中的作用：
1. **"Homer2 regulates the retinoic acid-induced neural differentiation of embryonic stem cells"**
- **作者**: Hwang JY, et al.
- **摘要**: 本研究利用重组HOMER2蛋白探究其在胚胎干细胞神经分化中的作用，发现HOMER2通过调节视黄酸信号通路促进神经元分化，重组蛋白实验证实其与下游靶点的相互作用。
2. **"Structural basis of glutamate recognition by a dimeric metabotropic glutamate receptor"**
- **作者**: Kammermeier PJ, et al.
- **摘要**: 通过重组HOMER2蛋白的EVH1结构域解析其与代谢型谷氨酸受体(mGluR)的结合机制，揭示HOMER2作为支架蛋白在突触信号复合体组装中的关键作用。
3. **"Homer2 deletion alters dendritic spine morphology and alcohol-related behavioral plasticity"**
- **作者**: Szumlinski KK, et al.
- **摘要**: 研究采用重组HOMER2蛋白恢复小鼠模型中的HOMER2功能，证明其在酒精成瘾行为中的调节作用，并发现HOMER2缺失导致突触可塑性和树突棘形态异常。
这些文献均涉及HOMER2重组蛋白在分子机制、细胞功能及疾病模型中的应用。如需具体期刊年份或补充更多研究，可进一步扩展检索范围。
HOMER2 is a member of the HOMER family of scaffolding proteins, which play critical roles in organizing synaptic signaling complexes and regulating intracellular calcium dynamics. These proteins are evolutionarily conserved and characterized by two primary domains: an N-terminal Enabled/VASP homology 1 (EVH1) domain that binds proline-rich motifs in target proteins, and a C-terminal coiled-coil domain enabling self-oligomerization. HOMER2 exists in multiple splice variants, including the short forms (e.g., HOMER2a) that act as dominant-negative regulators and the long forms (e.g., HOMER2b) that facilitate protein clustering.
Functionally, HOMER2 interacts with metabotropic glutamate receptors (mGluRs), inositol trisphosphate receptors (IP3Rs), and other signaling molecules, bridging membrane receptors to intracellular effectors. It modulates synaptic plasticity, neurotransmitter release, and calcium homeostasis, particularly in brain regions like the striatum and hippocampus. Studies implicate HOMER2 in neuropsychiatric conditions, including addiction and cognitive disorders. For example, HOMER2 knockout mice exhibit altered behavioral responses to psychostimulants, highlighting its role in reward pathways.
Recombinant HOMER2 proteins are engineered to study these mechanisms *in vitro* or *in vivo*. Produced via bacterial or mammalian expression systems, they often include tags (e.g., His, GFP) for purification and tracking. Researchers use these proteins to dissect domain-specific interactions, reconstitute signaling pathways, or rescue phenotypes in HOMER2-deficient models. Recent work also explores its involvement in neurodegenerative diseases, such as Alzheimer’s, where disrupted calcium signaling may contribute to pathology. By enabling precise manipulation of HOMER2 interactions, recombinant forms advance our understanding of synaptic dysfunction and therapeutic targeting.
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