| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOMER1 |
| Uniprot No | Q86YM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 氨基酸序列 | MGEQPIFSTRAHVFQIDPNTKKNWVPTSKHAVTVSYFYDSTRNVYRIISLDGSKAIINSTITPNMTFTKTSQKFGQWADSRANTVYGLGFSSEHHLSKFAEKFQEFKEAARLAKEKSQEKMELTSTPSQESAGGDLQSPLTPESINGTDDERTPDVTQNSEPRAEPTQNALPFSHSSAISKHWEAELATLKGNNAKLTAALLESTANVKQWKQQLAAYQEEAERLHKRVTELECVSSQANAVHTHKTELNQTIQELEETLKLKEEEIERLKQEIDNARELQEQRDSLTQKLQEVEIRNKDLEGQLSDLEQRLEKSQNEQEAFRNNLKTLLEILDGKIFELTELRDNLAKLLECS |
| 预测分子量 | 44.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HOMER1重组蛋白的3篇参考文献(简化版)，涵盖其结构、功能和疾病关联研究：
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1. **文献名称**：*Homer1a regulates the activity-induced synaptic scaling*
**作者**：Hu, J.H., et al.
**摘要**：研究利用重组HOMER1蛋白分析其在突触可塑性中的作用，发现HOMER1a通过调节mGluR信号通路影响神经元兴奋性，为神经疾病治疗提供靶点。
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2. **文献名称**：*Crystal structure of the Homer1 EVH1 domain and its interaction with polyproline motifs*
**作者**：Beneken, J., et al.
**摘要**：解析HOMER1重组蛋白EVH1结构域晶体结构，揭示其与富含脯氨酸基序的结合机制，阐明其在突触后信号复合体组装中的分子基础。
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3. **文献名称**：*Homer1 knockdown protects dopaminergic neurons in a Parkinson's disease model*
**作者**：Chen, X., et al.
**摘要**：通过体外重组HOMER1蛋白干预，发现抑制HOMER1可减轻帕金森模型中多巴胺神经元损伤，提示其作为神经退行性疾病潜在治疗靶点。
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*注：以上文献信息为示例性质，实际引用时建议通过PubMed或Web of Science核实具体细节。*
HOMER1 is a scaffolding protein encoded by the *HOMER1* gene, belonging to the HOMER family of postsynaptic density proteins. It plays a critical role in organizing synaptic signaling complexes by interacting with metabotropic glutamate receptors (mGluRs), inositol triphosphate receptors (IP3Rs), and other cytoskeletal elements. Structurally, HOMER1 contains an N-terminal Enabled/VASP homology 1 (EVH1) domain for binding proline-rich motifs in target proteins and a C-terminal coiled-coil domain enabling self-oligomerization. These features allow HOMER1 to form multivalent scaffolds that regulate calcium signaling, synaptic plasticity, and receptor trafficking.
Alternative splicing generates two major isoforms: the short, activity-inducible Homer1a and the long, constitutively expressed Homer1b/c. Homer1a lacks the coiled-coil domain, acting as a dominant-negative regulator that disrupts HOMER1-mediated complexes during neuronal activation. This dynamic regulation is crucial for adapting synaptic strength in learning and memory processes. Dysregulation of HOMER1 has been linked to neuropsychiatric disorders, including schizophrenia, addiction, and epilepsy, highlighting its therapeutic relevance.
Recombinant HOMER1 proteins are engineered for functional studies, typically expressed in bacterial or mammalian systems with tags (e.g., His, GST) for purification. These tools enable researchers to dissect protein-protein interactions, map binding domains, or screen small molecules targeting HOMER1-associated pathways. For instance, recombinant Homer1a is used to study mGluR5 signaling modulation in addiction models, while full-length isoforms help reconstitute synaptic complexes *in vitro*. Additionally, HOMER1 variants with mutations in EVH1 or coiled-coil domains are employed to explore structural determinants of scaffolding activity. Such studies advance our understanding of synaptic pathology and guide drug discovery for neurological diseases.
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